Narczewska B, Czyrski J A, Inglot A D
Can J Biochem Cell Biol. 1985 Mar;63(3):187-94. doi: 10.1139/o85-027.
Platelet-derived growth factor (PDGF) was isolated from platelets obtained from fresh bovine blood. The platelet lysates were extracted with acid-ethanol, according to the classic procedure that has been applied to the preparation of various protein hormones and transforming growth factors, but not until now to PDGF isolation. Bovine PDGF was further purified by ion-exchange chromatography on DEAE-Sephadex, CM-Sephadex, and finally by molecular sieving on Sephadex G-100. Bovine PDGF has an isoelectric point of 9.45-10.6 and a molecular weight ranging from 28 000 to 31 000. Reduction of bovine PDGF with 2-mercaptoethanol revealed the presence of two polypeptides with molecular weights of approximately 14 000 and 15 000. A simple biologic microassay of cell multiplication-stimulating activity has been developed in our laboratory. Purified bovine PDGF stimulated the proliferation of BALB/c 3T3 or human fibroblasts at minimal concentrations of 0.36-1.5 ng/mL.
血小板衍生生长因子（PDGF）是从新鲜牛血中获取的血小板中分离出来的。按照已应用于各种蛋白质激素和转化生长因子制备的经典程序，用酸乙醇提取血小板裂解物，但迄今为止尚未用于PDGF的分离。牛PDGF通过在DEAE-葡聚糖凝胶、CM-葡聚糖凝胶上进行离子交换色谱进一步纯化，最后通过葡聚糖凝胶G-100进行分子筛纯化。牛PDGF的等电点为9.45 - 10.6，分子量范围为28000至31000。用2-巯基乙醇还原牛PDGF显示存在两种分子量约为14000和15000的多肽。我们实验室已开发出一种简单的细胞增殖刺激活性生物微测定法。纯化的牛PDGF在最低浓度为0.36 - 1.5 ng/mL时刺激BALB/c 3T3或人成纤维细胞的增殖。
