MacKenzie M R, Lewis J P
Cancer Genet Cytogenet. 1985 May;17(1):13-20. doi: 10.1016/0165-4608(85)90096-2.
Multiple myeloma is traditionally thought of as a disease of plasma cells. Evidence from studies using antiodiotype antibodies, however, suggests that malignant events may take place in a precursor lymphocyte perhaps as early as the pre-B cell. In this study, we present cytogenetic evidence to support the latter view. Peripheral blood was obtained from a patient with plasma cell leukemia and light chain disease. Karyotypic analysis, using Giemsa banding techniques, showed an abnormal karyotype: 44,XY,-6,-8,-13,-16,-22,+mar1,+ mar2,+mar3,del(1)(p22,p32),11p+,13q+,14q+. A suspension culture was established and a plasma cell line was grown. It was characterized by transmission electron microscopy as having an eccentric nucleus, abundant cytoplasm, and extensive endoplasmic reticulum. A subculture of this line was subsequently grown that was characterized by transmission electron microscopy as a lymphoid cell with diminished quantity of cytoplasm without extensive endoplasmic reticulum. Karyotypic analysis of the smaller cell demonstrated a modal number of 88 chromosomes and was a tetraploid derivative of the first. Our study provides cytogenetic evidence that cells with a lymphocytic phenotype show karyotypic abnormalities seen in the malignant plasma cell of the same patient, and thus, can be considered as evidence favoring the initiating cell of plasma cell myeloma as being an early B lymphocyte.
多发性骨髓瘤传统上被认为是一种浆细胞疾病。然而，使用抗独特型抗体的研究证据表明，恶性事件可能早在前B细胞阶段就在前体淋巴细胞中发生。在本研究中，我们提供细胞遗传学证据来支持后一种观点。从一名患有浆细胞白血病和轻链病的患者获取外周血。使用吉姆萨显带技术进行核型分析，结果显示核型异常：44,XY,-6,-8,-13,-16,-22,+mar1,+ mar2,+mar3,del(1)(p22,p32),11p+,13q+,14q+。建立了悬浮培养并培养出一个浆细胞系。通过透射电子显微镜观察，其特征为具有一个偏心核、丰富的细胞质和广泛的内质网。随后对该细胞系进行传代培养，传代培养后的细胞通过透射电子显微镜观察为淋巴细胞，其细胞质数量减少且没有广泛的内质网。对较小细胞进行核型分析显示染色体众数为88条，是第一个细胞系的四倍体衍生物。我们的研究提供了细胞遗传学证据，表明具有淋巴细胞表型的细胞显示出与同一患者恶性浆细胞中所见相同的核型异常，因此，可以被视为支持浆细胞骨髓瘤起始细胞为早期B淋巴细胞的证据。
