Bayrak Büşra, Şen Uğur, Gökçek Dilek, Şirin Emre
Department of Agricultural Biotechnology, Faculty of Agriculture, Ondokuz Mayis University, 55139, Samsun, Türkiye.
Department of Agricultural Biotechnology, Faculty of Agriculture, Kırşehir Ahi Evran University, 40100, Kirsehir, Türkiye.
Trop Anim Health Prod. 2025 Jan 27;57(2):36. doi: 10.1007/s11250-025-04277-y.
The present study was conducted on specific skeletal muscles of six weaned male kids from each of the Angora, Hair, Honamlı, and Kilis goat breeds. The relationships between the expression of myogenic factor 5 (Myf5) and myogenic factor 6 (Myf6) genes and muscle fibre characteristics were analysed. Muscle samples from the longissimus dorsi (LD) and semitendinosus (ST) were collected from six 90-day-old weaned male kids of each breed. Muscle fiber characteristics were assessed through histochemical staining, while expression levels of Myf5 and Myf6 genes were quantified using real-time PCR. Total RNA content in the LD and ST muscles was significantly higher (p < 0.05) in Honamlı kids compared to those of the other breeds. Similarly, the expression of Myf5 gene in Honamlı kids was significantly higher (p < 0.05) than that observed in kids from the other breeds in LD muscle. Conversely, in the ST muscle, Hair kids exhibited a significantly lower expression of Myf5 (p < 0.05) when compared to both Honamlı and Kilis kids Additionally, Kilis kids demonstrated a significant reduced expression of Myf6 gene (p < 0.05) relative to the other breeds. The highest expression levels of the Myf6 gene (p < 0.05) were detected in the ST muscle of Honamlı and Angora kids, significantly surpassing those observed in Hair and Kilis kids. Moreover, significant correlations (p < 0.05) were observed among Myf5 and Myf6 gene expression levels and various muscle fiber characteristics differing across breeds. The results of this study underscore the pivotal role of these myogenic regulatory factors in muscle development, offering insights into the molecular mechanism driving breed-specific muscle growth. This association between gene expression and muscle phenotype could have profound implications for targeted breeding programs aimed at optimizing muscle traits in livestock species.
本研究对来自安哥拉、毛发、霍南姆利和基利斯山羊品种的6只断奶雄性幼羊的特定骨骼肌进行了研究。分析了生肌因子5（Myf5）和生肌因子6（Myf6）基因表达与肌纤维特征之间的关系。从每个品种的6只90日龄断奶雄性幼羊中采集背最长肌（LD）和半腱肌（ST）的肌肉样本。通过组织化学染色评估肌纤维特征，同时使用实时PCR对Myf5和Myf6基因的表达水平进行定量。与其他品种相比，霍南姆利幼羊的LD和ST肌肉中的总RNA含量显著更高（p<0.05）。同样，霍南姆利幼羊的Myf5基因在LD肌肉中的表达显著高于其他品种的幼羊（p<0.05）。相反，在ST肌肉中，与霍南姆利和基利斯幼羊相比，毛发幼羊的Myf5表达显著降低（p<0.05）。此外，基利斯幼羊的Myf6基因表达相对于其他品种显著降低（p<0.05）。在霍南姆利和安哥拉幼羊的ST肌肉中检测到Myf6基因的最高表达水平（p<0.05），显著超过毛发和基利斯幼羊。此外，在不同品种间，Myf5和Myf6基因表达水平与各种肌纤维特征之间存在显著相关性（p<0.05）。本研究结果强调了这些生肌调节因子在肌肉发育中的关键作用，为驱动品种特异性肌肉生长的分子机制提供了见解。基因表达与肌肉表型之间的这种关联可能对旨在优化家畜肌肉性状的定向育种计划具有深远意义。
