小鼠精子发生过程中精子细胞核糖体的超微结构和功能变化

Ultrastructural and functional variations in the spermatid nucleolus during spermiogenesis in the mouse.

作者信息

Mirre C, Knibiehler B

出版信息

Cell Differ. 1985 Feb;16(1):51-61. doi: 10.1016/0045-6039(85)90607-4.

Abstract

The localization, structure, and activity of the nucleolus-organizers (NORs) were studied during spermiogenesis in the mouse by light and electron microscopy procedures including NOR-silver-staining and actinomycin D treatment. After the two meiotic divisions the NORs resume their activity during the Golgi phase of spermatid differentiation (steps 1-3), and the nucleolus displays a specific 'padlock' structure containing the fibrillar components of an active nucleolus. This activity drops during the cap phase (steps 4-7) during which the nucleolus undergoes a segregation process of its components. No nucleolar structure is visible during the acrosomal and maturation phases of spermatid differentiation.

摘要

通过包括核仁组织区银染和放线菌素 D 处理在内的光学和电子显微镜程序，研究了小鼠精子发生过程中核仁组织区（NORs）的定位、结构和活性。在两次减数分裂之后，NORs 在精子细胞分化的高尔基期（第 1 - 3 步）恢复其活性，并且核仁呈现出一种特定的“挂锁”结构，其中包含活跃核仁的纤维成分。这种活性在帽期（第 4 - 7 步）下降，在此期间核仁经历其成分的分离过程。在精子细胞分化的顶体期和成熟期看不到核仁结构。