Jin Aofei, Tursun Dilbar, Tan Lirong, Yang Zhuonan, Duo Zhixian, Qin Yanan, Zhang Rui
Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi 830054, Xinjiang, China.
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science & Technology, Urumqi 830046, Xinjiang, China.
Genome. 2025 Jan 1;68:1-9. doi: 10.1139/gen-2024-0114.
Benzo(a)pyrene produced by food during high-temperature process enters the body through ingestion, which causes food safety issues to the human body. To alleviate the harm of foodborne benzo(a)pyrene to human health, a strain that can degrade benzo(a)pyrene was screened from Kefir, a traditional fermented product in Xinjiang. M72-4 is a Gram-positive bacteria sourced from Xinjiang traditional fermented product Kefir; under benzo(a)pyrene stress conditions, there was 69.39% degradation rate of 20 mg/L benzo(a)pyrene by strain M72-4 after incubation for 72 h. The whole genome of M72-4 was sequenced using PacBio sequencing technology in this study. The genome size was 5754 801 bp and a GC content was 35.24%; a total of 5719 coding genes were predicted bioinformatically. Through functional database annotation, it was found that the strain has a total of 219 genes involved in the transportation and metabolism of hydrocarbons, a total of 9 metabolic pathways related to the degradation and metabolism of exogenous substances, and a total of 67 coding genes. According to the Kyoto Encyclopedia of Genes and Genomes database annotation results, a key enzyme related to benzo(a)pyrene degradation, catechol 2,3-dioxygenase, was detected in the genome data of M72-4, encoding genes and , respectively. There are also monooxygenases and dehydrogenases. Therefore, it can be inferred that this strain mainly degrades benzo(a)pyrene through benzoate metabolic.
食品在高温加工过程中产生的苯并(a)芘通过摄入进入人体，从而引发食品安全问题。为减轻食源性苯并(a)芘对人体健康的危害，从新疆传统发酵制品开菲尔中筛选出一株可降解苯并(a)芘的菌株。M72-4是一株源自新疆传统发酵制品开菲尔的革兰氏阳性菌；在苯并(a)芘胁迫条件下，菌株M72-4在20mg/L苯并(a)芘中培养72h后降解率为69.39%。本研究采用PacBio测序技术对M72-4的全基因组进行测序。基因组大小为5754801bp，GC含量为35.24%；通过生物信息学预测共得到5719个编码基因。通过功能数据库注释发现，该菌株共有219个参与碳氢化合物运输和代谢的基因，共有9条与外源物质降解代谢相关的代谢途径，共有67个编码基因。根据京都基因与基因组百科全书数据库注释结果，在M72-4的基因组数据中检测到与苯并(a)芘降解相关的关键酶邻苯二酚2,3-双加氧酶，分别编码基因和。还存在单加氧酶和脱氢酶。因此，可以推断该菌株主要通过苯甲酸代谢途径降解苯并(a)芘。
