Wang Yuhui, Guo Hengke, Wan Wang, Jing Biao, Bai Yulong, Sun Jialu, Zhang Xin, Gao Zhenming, Liu Yu, Dong Xuepeng
The Second Hospital of Dalian Medical University, Dalian, 116023, China.
State Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Adv Healthc Mater. 2025 Mar;14(7):e2404713. doi: 10.1002/adhm.202404713. Epub 2025 Jan 28.
The intricate morphology, physicochemical properties, and interacting proteins of lipid droplets (LDs) are associated with cell metabolism and related diseases. To uncover these layers of information, a solvatochromic and photosensitized LDs-targeted probe based on the furan-based D-D-π-A scaffold is developed to offer the following integrated functions. First, the turn-on fluorescence of the probe upon selectively binding to LDs allows for direct visualization of their location and morphology. Second, its solvatochromic fluorescence with linear correlation to polarity quantifies micro-environmental heterogeneity among LDs. Third, the unique photosensitized properties enable photocatalytic proximity labeling and enrichment of LDs-interacting proteins, ready for potential downstream proteomic analysis. These functions are exemplified using artificial LDs in buffer, stressed liver cell line, and diseased liver tissues biopsied from patients. While most LD sensors only offer fluorescence imaging functions, the multi-functional LD probe reported herein integrates both singlet fluorescence and triplet photosensitization properties for LDs studies.
脂滴（LDs）复杂的形态、物理化学性质及相互作用蛋白与细胞代谢和相关疾病有关。为揭示这些信息层面，基于呋喃基D-D-π-A支架开发了一种具有溶剂化显色和光敏特性的靶向LDs的探针，以提供以下综合功能。首先，该探针在选择性结合LDs时开启荧光，可直接观察其位置和形态。其次，其与极性呈线性相关的溶剂化显色荧光可量化LDs之间的微环境异质性。第三，独特的光敏特性能够实现光催化邻近标记和富集与LDs相互作用的蛋白，为潜在的下游蛋白质组学分析做好准备。在缓冲液中的人工LDs、应激肝细胞系以及取自患者的患病肝脏组织中对这些功能进行了验证。虽然大多数LD传感器仅提供荧光成像功能，但本文报道的多功能LD探针整合了单重态荧光和三重态光敏特性用于LDs研究。
