Liu Yu, Mu Hongbei, Chen Yu, Li Kexin, Mei Qiaojuan, Wang Lingjuan, Tang Tianyu, Shen Qiuzi, Li Huaibiao, Zhang Ling, Li Jing, Xiang Wenpei
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Department of Gynaecology, Hubei Maternal and Child Health Hospital, Wuhan 430070, China.
Life Med. 2024 Mar 25;3(1):lnae013. doi: 10.1093/lifemedi/lnae013. eCollection 2024 Feb.
Ovarian aging is mainly characterized by a progressive decline in oocyte quantity and quality, which ultimately leads to female infertility. Various therapies have been established to cope with ovarian aging, among which exosome-based therapy is considered a promising strategy that can benefit ovarian functions via multiple pathways. Here, we isolated and characterized exosomes derived from ovarian follicular fluid and profiled the differential expression patterns of noncoding exosomal RNAs in young and aged women. Treatment with young mouse-derived exosomes efficiently rescued ovarian function in aged mice. The follicular fluid exosomes from young mice and miR-320-3p can also promote the proliferation of ovarian granulosa cells and improve mitochondrial function from old mice . The mechanism may be involve that exosomes transfer miR-320-3p to granulosa cells, and inhibit the expression of FOXQ1. Exosomes also can increase the number of primordial and growing follicles, and improve the developmental ability of oocytes in the old mice . And hnRNPA2B1 controls miR-320-3p entry into exosomes. This work provides insights into the antiaging potential of follicular fluid-derived exosomes and the underlying molecular mechanisms, which may facilitate prevention of ovarian aging and an improvement in female fertility.
卵巢衰老的主要特征是卵母细胞数量和质量的逐渐下降，最终导致女性不孕。人们已经建立了各种疗法来应对卵巢衰老，其中基于外泌体的疗法被认为是一种有前景的策略，它可以通过多种途径使卵巢功能受益。在此，我们分离并鉴定了源自卵巢卵泡液的外泌体，并分析了年轻和老年女性非编码外泌体RNA的差异表达模式。用年轻小鼠来源的外泌体处理可有效挽救老年小鼠的卵巢功能。来自年轻小鼠的卵泡液外泌体和miR-320-3p也可以促进卵巢颗粒细胞的增殖，并改善老年小鼠的线粒体功能。其机制可能涉及外泌体将miR-320-3p转移至颗粒细胞，并抑制FOXQ1的表达。外泌体还可以增加老年小鼠原始卵泡和生长卵泡的数量，并提高卵母细胞的发育能力。并且hnRNPA2B1控制miR-320-3p进入外泌体。这项工作为卵泡液来源外泌体的抗衰老潜力及其潜在分子机制提供了见解，这可能有助于预防卵巢衰老并改善女性生育能力。
