Tadesse Mahlet Tewodros, Ayalke Libse Lemma, Tedla Beza Fitiwi, Weldemeskel Fikrite Adefris, Yirdaw Ahmed Seid, Abebe Tsegahun Asfaw
Department of Medical Laboratory Science, College of Health Science Debre Berhan University Debre Berhan Ethiopia.
Health Sci Rep. 2025 Jan 27;8(1):e70361. doi: 10.1002/hsr2.70361. eCollection 2025 Jan.
Clinical laboratories play a vital role in modern healthcare, where laboratory test results significantly influence medical decisions. However, laboratory professionals are often exposed to hazardous biological materials, underscoring the importance of biosafety management. This study aimed to assess biosafety practices among clinical laboratory professionals in Debre Berhan town, Ethiopia.
A cross-sectional study involving 81 participants was conducted using a standard questionnaire covering work habits, protective barriers, safety equipment, and biosafety policy perception. Descriptive data was summarized using a table and graph. Logistic regression was employed to determine factors affecting adherence to biosafety protocols. Statistically significant value was identified using value ≤ 0.05, 95% CI, and OR.
The results revealed high compliance with essential work habits, such as the use of mechanical pipettes 77 (95.1%) and proper handwashing after handling biohazardous material 78 (96.3%) and after removing gloves 80 (98.8%). However, adherence to the use of protective barriers 50 (61.7%), biosafety training 15 (18.5%), and emergency preparedness 28 (34.6%) were notably low. Only 35 (43.2%) of participants received appropriate immunizations, and access to biosafety equipment like biological safety cabinets was limited 15 (18.5%). Experience and training were significant predictors of biosafety compliance, highlighting the need for continuous education and institutional support.
The findings underscore the importance of enhancing training programs, improving access to safety equipment, and ensuring the consistent application of biosafety protocols to protect laboratory personnel from hazardous biological materials and maintain public health.
临床实验室在现代医疗保健中发挥着至关重要的作用，实验室检测结果对医疗决策有着重大影响。然而，实验室专业人员经常接触到危险的生物材料，这凸显了生物安全管理的重要性。本研究旨在评估埃塞俄比亚德布雷伯汉镇临床实验室专业人员的生物安全实践情况。
采用横断面研究，涉及81名参与者，使用涵盖工作习惯、防护屏障、安全设备和生物安全政策认知的标准问卷。描述性数据用表格和图表进行总结。采用逻辑回归确定影响生物安全协议遵守情况的因素。使用P值≤0.05、95%置信区间和比值比确定具有统计学意义的值。
结果显示，在基本工作习惯方面，如使用机械移液器的有77人（95.1%），处理生物危险材料后正确洗手的有78人（96.3%），摘掉手套后正确洗手的有80人（98.8%），依从性较高。然而，防护屏障的使用依从性为50人（61.7%），生物安全培训的依从性为15人（18.5%），应急准备的依从性为28人（34.6%），明显较低。只有35人（43.2%）的参与者接受了适当的免疫接种，生物安全柜等生物安全设备的获取有限，为15人（18.5%）。经验和培训是生物安全合规性的重要预测因素，凸显了持续教育和机构支持的必要性。
研究结果强调了加强培训计划、改善安全设备获取以及确保生物安全协议的一致应用的重要性，以保护实验室人员免受危险生物材料的侵害并维护公众健康。
