Tang Mei, Feng Jianru, Wang Xudong, Ding Yu
Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institute, Zhanjiang, China.
Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institute, Zhanjiang, China.
Vet Immunol Immunopathol. 2025 Feb;280:110887. doi: 10.1016/j.vetimm.2025.110887. Epub 2025 Jan 24.
Vibrio alginolyticus is a serious aquaculture bacterial pathogen, which is widely distributed in the ocean and rivers, and cause vibriosis in aquaculture. Therefore, it is imperative to develop effective vaccine to prevent vibriosis. In this study, the efficacy of gr deletion strain (Δgr) of V. alginolyticus as an attenuated live vaccine was evaluated in peal gentian grouper by intraperitoneal injection immunization, followed by a wild strain injection challenge. Real-time fluorescence quantitative PCR was used for analyzing the expression dynamics of immune-related genes. ELISA was used to assess the serum antibody titer and non-specific enzyme activities were determined by kit. The results showed that the safe dose of Δgr was 1.0 × 10 CFU/mL for pearl gentian grouper, and the immune protection rate of Δgr was 73.08 %, providing significant protection against V. alginolyticus. The expression levels of all the immune-related genes MHC-Iα, TNF-α, IL-1β, IL-16 and MyD88 were upregulated in liver, spleen, head kidney and thymus within 42 days post-vaccination. In addition, specific antibody IgM, activities of catalase, superoxide dismutase and lysozyme were also significantly increased compared with the control group. It is not only safe for groupers, but also can effectively stimulate both specific and non-specific immunity of fish. These results indicated that Δgr could be used as an effective attenuated live vaccine candidate to prevent pearl gentian grouper against V. alginolyticus in aquaculture.
溶藻弧菌是一种严重的水产养殖细菌性病原菌，广泛分布于海洋和河流中，可引起水产养殖中的弧菌病。因此，开发有效的疫苗来预防弧菌病势在必行。在本研究中，通过腹腔注射免疫，然后进行野生菌株注射攻毒，评估了溶藻弧菌gr缺失菌株（Δgr）作为减毒活疫苗在珍珠龙胆石斑鱼中的效果。采用实时荧光定量PCR分析免疫相关基因的表达动态。采用ELISA法检测血清抗体效价，试剂盒测定非特异性酶活性。结果表明，Δgr对珍珠龙胆石斑鱼的安全剂量为1.0×10 CFU/mL，免疫保护率为73.08%，对溶藻弧菌具有显著的保护作用。接种疫苗后42天内，肝脏、脾脏、头肾和胸腺中所有免疫相关基因MHC-Iα、TNF-α、IL-1β、IL-16和MyD88的表达水平均上调。此外，与对照组相比，特异性抗体IgM、过氧化氢酶、超氧化物歧化酶和溶菌酶的活性也显著增加。它不仅对石斑鱼安全，而且能有效刺激鱼类的特异性和非特异性免疫。这些结果表明，Δgr可作为一种有效的减毒活疫苗候选物，用于预防珍珠龙胆石斑鱼在水产养殖中感染溶藻弧菌。
