El-Refaiy Amal I, Amer Nahed S, Alhejely Amani, Qahl Safa H, Shaban Amira M, Mohamed Amro E, Saleh Amira A, Badawy Abdelnaser A, El-Magd Mohammed A
Department of Agricultural Zoology and Nematology, Faculty of Agriculture (Girls), Al-Azhar University, Egypt.
Department of Biology, University College in Darb, Jazan University, Al-Darb, Jazan 45142, Saudi Arabia.
Mol Biochem Parasitol. 2025 Jun;262:111673. doi: 10.1016/j.molbiopara.2025.111673. Epub 2025 Jan 26.
This study investigated the effect of dandelion (Taraxacum officinale) leaf aqueous extract (DLE) on the immunological response of mice following infection with Schistosoma mansoni. Mice (in groups of 7) were first experimentally infected with S. mansoni and, 6 weeks later, were treated with praziquantel (PZQ) and/or DLE. Control mice were uninfected. In contrast to the untreated group, animals given PZQ and/or DLE exhibited an enhanced immunological response, as indicated by increased serum IFNγ, TNFα, IL4 and IL10 levels, increased numbers of CD4 + and CD25 + cells in blood and spleen and altered expression of apoptosis-related genes (low Bax and caspase3 and high Bcl2) in the spleen. DLE treatment had a significantly bigger impact in all these parameters compared with PZQ alone and combined DLE/PZQ treatment have the largest effect. While DLE treatment alone significantly decreased parasite burden, it did not improve upon the greater protective effect of PZQ, even when given in combination.
本研究调查了蒲公英（药用蒲公英）叶水提取物（DLE）对曼氏血吸虫感染后小鼠免疫反应的影响。将小鼠（每组7只）首先进行曼氏血吸虫实验性感染，6周后，用吡喹酮（PZQ）和/或DLE进行治疗。对照小鼠未感染。与未治疗组相比，给予PZQ和/或DLE的动物表现出增强的免疫反应，血清IFNγ、TNFα、IL4和IL10水平升高、血液和脾脏中CD4 +和CD25 +细胞数量增加以及脾脏中凋亡相关基因表达改变（低Bax和caspase3以及高Bcl2）表明了这一点。与单独使用PZQ相比，DLE治疗对所有这些参数的影响明显更大，DLE/PZQ联合治疗效果最大。虽然单独使用DLE治疗可显著降低寄生虫负荷，但即使联合使用，也未比PZQ的更大保护作用有所改善。
