Zhang Fan, Zheng Xujiao, Ma Ying, Nan Wuqiang, Wu Wenjing, Chu Ziru, Sun Xin, Huang Jihong, Muratkhan Marat, Yue Fangfang, Wang Xin, Lü Xin
College of Food Science and Engineering, Shaanxi Engineering Research Centre of Dairy Products Quality, Safety and Health, Northwest A&F University, Yangling 712100, Shaanxi, China.
Department of Pediatrics, Xijing Hospital, the Fourth Military Medical University. No. 127, Changle West Road, Xi'an, Shaanxi 710032, China.
Int J Biol Macromol. 2025 Apr;300:140375. doi: 10.1016/j.ijbiomac.2025.140375. Epub 2025 Jan 26.
The probiotic encapsulation system has the potential to enhance the prebiotic effects of probiotics. However, challenges arise from the release behavior of this system in vivo and the large size of hydrogel beads. This study aims to address the issues related to the size of previous hydrogel beads and assess the colon-targeted delivery of probiotic polysaccharides composite hydrogel beads (PPHB). PPHB prepared by gas-shearing technique significantly reduced the average particle size and demonstrated a high protective capacity for probiotics (after simulating intestinal conditions for 4 h, the viability of encapsulated probiotics remained at 10 CFU/g). The use of indocyanine green along with near-infrared-II in vivo imaging technology demonstrated the colon-targeted delivery of PPHB in vivo, which also extended the retention time of probiotics in the cecum and colon. Additionally, the colon-targeted delivery of PPHB was also demonstrated by dietary supplementation in vivo. PPHB significantly enhanced the diversity and richness of intestinal microflora species, increased the levels of short-chain fatty acids, raised the relative abundance of beneficial bacteria, and significantly decreased the relative abundance of harmful bacteria. Alginate-based PPHB is more suitable for encapsulating functional ingredients for colon-targeted delivery and modulating gut microbiota.
益生菌封装系统有增强益生菌益生元效应的潜力。然而，该系统在体内的释放行为以及水凝胶珠粒尺寸较大带来了挑战。本研究旨在解决与先前水凝胶珠粒尺寸相关的问题，并评估益生菌多糖复合水凝胶珠粒（PPHB）的结肠靶向递送。通过气体剪切技术制备的PPHB显著降低了平均粒径，并对益生菌表现出高保护能力（在模拟肠道条件4小时后，封装益生菌的存活率保持在10 CFU/g）。吲哚菁绿与近红外二区体内成像技术的联用证明了PPHB在体内的结肠靶向递送，这也延长了益生菌在盲肠和结肠中的停留时间。此外，通过体内膳食补充也证明了PPHB的结肠靶向递送。PPHB显著增强了肠道微生物群落物种的多样性和丰富度，提高了短链脂肪酸水平，增加了有益细菌的相对丰度，并显著降低了有害细菌的相对丰度。基于海藻酸盐的PPHB更适合封装用于结肠靶向递送和调节肠道微生物群的功能成分。
