雄性大鼠白色脂肪组织中依赖烟酰胺腺嘌呤二核苷酸磷酸氧化酶的活性氧生成增加以及抗氧化反应相应增强。
Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats.
作者信息
Nascimento Jessica de Freitas, de Oliveira Keciany Alves, de Freitas Paula Alexandre, Falci Júlia de Araújo Marques, Vasconcelos Renata Prado, Magalhães Saulo Chaves, Farias Talita Mendes, Alonso-Vale Maria Isabel Cardoso, Loureiro Adriano Cesar Carneiro, de Carvalho Denise Pires, Fortunato Rodrigo Soares, de Oliveira Ariclécio Cunha
机构信息
Universidade Estadual do Ceará Instituto Superior de Ciências Biomédicas Laboratório de Fisiologia Endócrina e Metabolismo FortalezaCE Brasil Laboratório de Fisiologia Endócrina e Metabolismo, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
Universidade Estadual do Ceará Programa de Pós-graduação em Nutrição e Saúde FortalezaCE Brasil Programa de Pós-graduação em Nutrição e Saúde, Universidade Estadual do Ceará, Fortaleza, CE, Brasil.
出版信息
Arch Endocrinol Metab. 2024 Nov 6;68(Spec Issue):e240136. doi: 10.20945/2359-4292-2024-0136. eCollection 2024.
OBJECTIVE
This study aimed to investigate the redox balance in subcutaneous and retroperitoneal fat pads of male and female Wistar rats.
MATERIALS AND METHODS
The study analyzed the activity and gene expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, along with the production of NADPH oxidases dependent on HO and gene expression of NOX1, NOX2, and NOX4.
RESULTS
The retroperitoneal fat pad in males compared with females had greater NOX2 and NOX4 expression, along with higher superoxide dismutase activity. Additionally, their subcutaneous fat pad had greater NOX4 expression and higher intracellular HO production, together with greater expression and activity of both superoxide dismutase and catalase.
CONCLUSION
The white adipose tissue of male rats had greater reactive oxygen species (ROS) production compared with that of female rats, but also a proportionally greater antioxidant response. These findings are important for ongoing investigations into how sex differences may be linked to the development of metabolic diseases and the unique susceptibilities of each sex.
目的
本研究旨在调查雄性和雌性Wistar大鼠皮下及腹膜后脂肪垫中的氧化还原平衡。
材料与方法
该研究分析了抗氧化酶超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性及基因表达，以及依赖于HO的NADPH氧化酶的产生和NOX1、NOX2及NOX4的基因表达。
结果
与雌性相比，雄性大鼠的腹膜后脂肪垫中NOX2和NOX4表达更高，超氧化物歧化酶活性也更高。此外，其皮下脂肪垫中NOX4表达更高，细胞内HO产生更多，同时超氧化物歧化酶和过氧化氢酶的表达及活性也更高。
结论
与雌性大鼠相比，雄性大鼠的白色脂肪组织产生的活性氧（ROS）更多，但抗氧化反应也相应更强。这些发现对于正在进行的关于性别差异如何与代谢疾病的发展以及两性各自独特易感性相关的研究具有重要意义。
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