Dai Qi, Lee Hsi-Ming, Giordano Austin, Chiang Fu-Pen, Walker Stephen G, Delgado-Ruiz Rafael, Johnson Francis, Golub Lorne M, Gu Ying
Department of General Dentistry, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United States.
Department of Oral Biology and Pathology, School of Dental Medicine, Stony Brook University, Stony Brook, NY, United States.
Front Oral Health. 2025 Jan 14;5:1506616. doi: 10.3389/froh.2024.1506616. eCollection 2024.
Previous studies have shown () esterase is a key mediator of dental composite biodegradation, which can contribute to recurrent caries. This study is to investigate the inhibitory effects of a novel Chemically-Modified-Curcumin (CMC 2.24) on esterase activities and related dental material biodegradation.
Dental adhesive materials and composite resins were incubated in suspension with CMC 2.24 and other compounds, including doxycycline, Chemically-Modified-Tetracycline (CMT-3), and curcumin for 4 weeks. The pre- and post-incubation surface roughness were evaluated by either laser diffraction pattern and/or a 3D laser scanning microscope. Esterase enzyme inhibition assays were performed with the same test groups and activities were determined spectrophotometrically.
Among all experimental groups, CMC 2.24 significantly reduced surface roughness of dental composite ( < 0.01) and adhesive ( < 0.01) materials compared to bacteria-only group. Additionally, CMC 2.24 reduced porcine esterase activity by 46.5%, while other compounds showed minimal inhibition. In the esterase assay, CMC 2.24 showed inhibition of 70.0%, while other compounds showed inhibition ranging from 19% to 36%.
Our study demonstrated that CMC 2.24 inhibited biodegradation of dental composite material more effectively than its mother compound, curcumin. Moreover, the mechanism of this biodegradation was likely mediated through bacterial esterase activity. Doxycycline achieved similar inhibition by completely eradicating with its antibiotic action; hence, it is not recommended for long-term use.
先前的研究表明（）酯酶是牙科复合材料生物降解的关键介质，这可能导致龋齿复发。本研究旨在探讨新型化学修饰姜黄素（CMC 2.24）对酯酶活性及相关牙科材料生物降解的抑制作用。
将牙科粘结材料和复合树脂与CMC 2.24及其他化合物（包括强力霉素、化学修饰四环素（CMT - 3）和姜黄素）在悬浮液中孵育4周。通过激光衍射图案和/或三维激光扫描显微镜评估孵育前后的表面粗糙度。对相同测试组进行酯酶抑制试验，并通过分光光度法测定活性。
在所有实验组中，与仅含细菌的组相比，CMC 2.24显著降低了牙科复合材料（<0.01）和粘结材料（<0.01）的表面粗糙度。此外，CMC 2.24使猪酯酶活性降低了46.5%，而其他化合物的抑制作用最小。在酯酶测定中，CMC 2.24的抑制率为70.0%，而其他化合物的抑制率在19%至36%之间。
我们的研究表明，CMC 2.24比其母体化合物姜黄素更有效地抑制牙科复合材料的生物降解。此外，这种生物降解的机制可能是通过细菌酯酶活性介导的。强力霉素通过其抗生素作用完全根除细菌而实现类似的抑制作用；因此，不建议长期使用。
