Liu Qian, Wu Lianzhi
Department of Obstetrics, Renmin Hospital of Wuhan University, Wuhan, China, China.
Ginekol Pol. 2025 Jan 29. doi: 10.5603/gpl.99058.
BJECTIVES: Circular RNAs (circRNAs) are known to be associated with the progression of gestational diabetes mellitus (GDM). Thus, the objective of this study was to unveil the influnce and potential mechanism of hsa_circ_0002768 in GDM. M: ATERIAL AND: METHODS: Levels of hsa_circ_0002768 were quantified by RT-qPCR. Placental hsa_circ_0002768 levels were analyzed after pregnancies. Trophoblast cell (HTR-8/SVneo) functions, including oxidative stress, mitochondrial dysfunction, cell viability, autophagy, and invasion, were evaluated upon hsa_circ_0002768 knockdown. Finally, the downstream miRNA for hsa_circ_0002768 was investigated. RESULTS: Hsa_circ_0002768 levels increased as high glucose-induced, and in GDM placenta. In vitro experiments showed that hsa_circ_0002768 knockdown positively regulated trophoblast oxidative stress and mitochondrial functions, thus inducing cell viability and invasion, but inhibiting autophagy. miR-339-5p was a downstream molecular for hsa_circ_0002768, which targeted to TLE3. CONCLUSIONS: This study reveals a physiological role for hsa_circ_0002768 during GDM.
已知环状RNA（circRNAs）与妊娠期糖尿病（GDM）的进展相关。因此，本研究的目的是揭示hsa_circ_0002768在GDM中的影响及潜在机制。
通过RT-qPCR定量hsa_circ_0002768的水平。在妊娠后分析胎盘hsa_circ_0002768水平。在hsa_circ_0002768敲低后评估滋养层细胞（HTR-8/SVneo）的功能，包括氧化应激、线粒体功能障碍、细胞活力、自噬和侵袭。最后，研究hsa_circ_0002768的下游miRNA。
hsa_circ_0002768水平在高糖诱导时以及GDM胎盘中升高。体外实验表明，hsa_circ_0002768敲低正向调节滋养层氧化应激和线粒体功能，从而诱导细胞活力和侵袭，但抑制自噬。miR-339-5p是hsa_circ_0002768的下游分子，其靶向TLE3。
本研究揭示了hsa_circ_0002768在GDM期间的生理作用。
