Pereira Débora Francine Gomes Silva, Nietsche Silvia, Xavier Adelica Aparecida, Mercadante-Simões Maria Olívia, Pereira Marlon Cristian Toledo
Departamento de Ciências Agrárias, Instituto Federal de Educação, Ciência e Tecnologia Baiano, Campus Bom Jesus da Lapa BR 349, Km 14 - Zona Rural, Bom Jesus da Lapa, BA, CEP: 47600-000, Brazil.
Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Avenida Universitária, 1000, Bairro Universitário, Montes Claros, MG, 39404-547, Brazil.
Braz J Microbiol. 2025 Jun;56(2):1277-1282. doi: 10.1007/s42770-025-01617-w. Epub 2025 Jan 29.
The objective of this work was to investigate the biofilm production capacity of the isolate EB-40 (Bacillus cereus) in a culture medium for the multiplication of microorganisms and in roots of in vitro grown banana explants. It was observed that the isolate was able to produce biofilms in tryptone, soy and agar (TSA) culture medium and in the roots of explants. The format, architecture and location of the biofilms in TSA culture medium presented an exopolymer matrix formed by EB-40 presented coccoid bacillary cells and fibrillar structures. In roots explants was verified the formation of microcolonies adhered to the root hairs. The information obtained in this experiment allowed inferring the ability of the isolate to produce biofilms and the colonization pattern shown when associated with banana tree roots.
这项工作的目的是研究分离株EB - 40(蜡样芽孢杆菌)在用于微生物增殖的培养基以及体外培养的香蕉外植体根中的生物膜形成能力。观察到该分离株能够在胰蛋白胨大豆琼脂(TSA)培养基和外植体根中产生生物膜。TSA培养基中生物膜的形态、结构和位置呈现出由EB - 40形成的胞外聚合物基质,其中有球状杆菌细胞和纤维状结构。在外植体根中证实形成了附着在根毛上的微菌落。该实验获得的信息有助于推断该分离株产生生物膜的能力以及与香蕉树根相关时所呈现的定殖模式。