Xu Ningning, Li Liping, Ippolito James A, Xing Weiqin, Wang Yale, Zhang Baozhong, Yang Yongqiang, Bai Ling, Zhao Linlin
College of Civil Engineering, Henan University of Technology, Zhengzhou, 450001, Henan, China.
School of the Environment, Henan University of Technology, Zhengzhou, 450001, Henan, China.
Biol Trace Elem Res. 2025 Jan 29. doi: 10.1007/s12011-025-04532-1.
The effect of heavy metal availability and interaction in feed on feces heavy metal excretion in mice has rarely been investigated. In this work, feed containing a polluted soil (total Cd = 6.34, total Pb = 387 mg kg) amended with phosphate, bentonite and lime, or feed spiked with soluble Pb and Cd were fed to mice for 10 days. Feces were collected on Day 2 and Day 10 and analyzed for Cd, Cu, Mn, Ni, Pb and Zn concentrations. Results indicated that Day 10 samples had 10% greater heavy metal concentrations in some treatments than Day 2. For the Pb and Cd spiked treatments, significant positive correlations were found between Pb and Cd rates and feces Pb and Cd concentrations on both dates. Significantly greater feces Pb and Cd concentrations were found in Day 10 samples than in Day 2 samples. Significant correlations were also found between feed Pb and Cd spiking rates and feces Cu, Mn, Ni and Zn concentrations, indicating mouse metal absorption dynamics and metal interactions. Although the phosphate and lime amended soils had lower DTPA-Pb and Cd than the unamended soil (6.2-17.9%, p < 0.05), no significant difference was found for feces Pb and Cd concentrations between treatments. A marginally negative correlation (p = 0.073) between soil DTPA-Zn and feces Zn concentration for Day 2 samples implied the soil amendment only affected mice absorption of Zn. Overall results imply that mice feces heavy metal concentrations can be used to indicate heavy metal absorption from feed and metal interactions in mice.
饲料中重金属有效性及相互作用对小鼠粪便重金属排泄的影响鲜有研究。本研究中，将添加了磷酸盐、膨润土和石灰的污染土壤（总镉=6.34，总铅=387mg/kg）的饲料或添加了可溶性铅和镉的饲料喂给小鼠10天。在第2天和第10天收集粪便，分析镉、铜、锰、镍、铅和锌的浓度。结果表明，在某些处理中，第10天的样品中重金属浓度比第2天高10%。对于添加铅和镉的处理，在两个日期的铅和镉添加率与粪便中铅和镉浓度之间均发现显著正相关。第10天样品中的粪便铅和镉浓度显著高于第2天样品。在饲料铅和镉添加率与粪便铜、锰、镍和锌浓度之间也发现显著相关性，表明小鼠的金属吸收动态和金属相互作用。尽管添加了磷酸盐和石灰的土壤中DTPA-铅和镉含量低于未改良土壤（6.2-17.9%，p<0.05），但各处理之间粪便铅和镉浓度未发现显著差异。第2天样品中土壤DTPA-锌与粪便锌浓度之间存在微弱负相关（p=0.073），这意味着土壤改良仅影响小鼠对锌的吸收。总体结果表明，小鼠粪便中的重金属浓度可用于指示小鼠从饲料中吸收的重金属及金属相互作用情况。
