Kirimlioglu Esma, Katirci Ertan, Simsek Mehmet
Departments of Histology and Embryology, School of Medicine, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Faculty of Medicine, Department of Histology and Embryology, Ahi Evran University, Kırşehir, Turkey.
Histochem Cell Biol. 2025 Jan 29;163(1):29. doi: 10.1007/s00418-024-02351-4.
Preeclampsia (PE) is a severe placental complication occurring after the 20th week of pregnancy. PE is associated with inflammation and an increased immune reaction against the fetus. TYRO3 and PROS1 suppress inflammation by clearing apoptotic cells. Disruptions in TYRO3/PROS1 signaling may increase the risk of PE. This study investigated the role of TYRO3/PROS1 signaling in the development of PE using healthy placentae (HP) and preeclamptic placentae (PP) of six pregnant women each. Tissue morphology using hematoxylin and eosin (H&E), TYRO3, MERTK, PROS1, and GAS6 mRNA levels using qPCR and localization and expression levels of TYRO3 and PROS1 using immunohistochemical staining (IHC) were evaluated. The study results show that the levels of TYRO3, MERTK, PROS1 and GAS6 mRNA, as well as TYRO3 protein, increased in PE. TYRO3 expression was observed in extravillous trophoblast (EVTs) and syncytiotrophoblast cells (SCTs). PROS1 was observed in HP fetal vessels through IHC while absent in PP. The reduced presence of PROS1 in the cytotrophoblast layer in PE may indicate a compromised blood-placental barrier. The absence of PROS1 in fetal vessels may suggest potential complement activation and thrombosis. TYRO3, MERTK, PROS1 and GAS6 may help balance impaired inflammation, apoptosis, thrombosis, complement activation and the blood-placental barrier in PE.
子痫前期（PE）是一种发生在妊娠20周后的严重胎盘并发症。PE与炎症以及针对胎儿的免疫反应增强有关。TYRO3和PROS1通过清除凋亡细胞来抑制炎症。TYRO3/PROS1信号通路的破坏可能会增加患PE的风险。本研究使用6名孕妇的健康胎盘（HP）和子痫前期胎盘（PP），调查了TYRO3/PROS1信号通路在PE发生发展中的作用。通过苏木精和伊红（H&E）染色评估组织形态，使用qPCR评估TYRO3、MERTK、PROS1和GAS6的mRNA水平，并使用免疫组织化学染色（IHC）评估TYRO3和PROS1的定位及表达水平。研究结果显示，PE中TYRO3、MERTK、PROS1和GAS6的mRNA水平以及TYRO3蛋白水平均升高。在绒毛外滋养层细胞（EVT）和合体滋养层细胞（SCT）中观察到TYRO3表达。通过IHC在HP胎儿血管中观察到PROS1，而在PP中未观察到。PE中细胞滋养层中PROS1的减少可能表明血 - 胎盘屏障受损。胎儿血管中PROS1的缺失可能提示潜在的补体激活和血栓形成。TYRO3、MERTK、PROS1和GAS6可能有助于平衡PE中受损的炎症、细胞凋亡、血栓形成、补体激活和血 - 胎盘屏障。
