Amado Douglas, Koch Eva L, Cordeiro Erick M G, Araújo Wellingson A, Garcia Antonio A F, Heckel David G, Montejo-Kovacevich Gabriela, North Henry L, Corrêa Alberto S, Jiggins Chris D, Omoto Celso
Department of Zoology, University of Cambridge, Cambridge, United Kingdom.
Department of Entomology and Acarology, Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba, São Paulo, Brazil.
PLoS One. 2025 Jan 29;20(1):e0318154. doi: 10.1371/journal.pone.0318154. eCollection 2025.
Insecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera is a globally distributed crop pest affecting over 300 crop species. H. armigera has rapidly evolved insecticide resistance, making it one of the most damaging pests worldwide. Understanding the genetic basis of insecticide resistance provides insights to develop tools, such as molecular markers, that can be used to slow or prevent the evolution of resistance. We explore the genetic architecture of H. armigera resistance to a widely used insecticide, flubendiamide, using two complementary approaches: genome-wide association studies (GWAS) in wild-caught samples and quantitative trait locus (QTL) mapping in a controlled cross of susceptible and resistant laboratory strains. Both approaches identified one locus on chromosome 2, revealing two SNPs within 976 bp that can be used to monitor field resistance to flubendiamide. This was the only region identified using linkage mapping, though GWAS revealed additional sites associated with resistance. Other loci identified by GWAS in field populations contained known insecticide detoxification genes from the ATP-binding cassette family, ABCA1, ABCA3, ABCF2 and MDR1. Our findings revealed an oligogenic genetic architecture, contrasting previous reports of monogenic resistance associated with the ryanodine receptor. This work elucidates the genetic basis of rapidly evolving insecticide resistance and will contribute to developing effective insecticide resistance management strategies.
抗药性是粮食生产、环境可持续性和人类健康领域的一个重大问题。棉铃虫是一种分布于全球的作物害虫，会影响300多种作物。棉铃虫已迅速进化出抗药性，成为全球最具破坏力的害虫之一。了解抗药性的遗传基础有助于开发如分子标记等工具，用于减缓或防止抗药性的进化。我们采用两种互补方法，探究棉铃虫对广泛使用的杀虫剂氟苯虫酰胺的抗性遗传结构：对野外捕获样本进行全基因组关联研究（GWAS），以及在敏感和抗性实验室品系的受控杂交中进行数量性状位点（QTL）定位。两种方法均在2号染色体上确定了一个位点，在976 bp范围内发现了两个单核苷酸多态性（SNP），可用于监测田间对氟苯虫酰胺的抗性。这是通过连锁图谱定位确定的唯一区域，不过GWAS还揭示了其他与抗性相关的位点。GWAS在田间种群中鉴定出的其他位点包含来自ATP结合盒家族的已知杀虫剂解毒基因，ABCA1、ABCA3、ABCF2和MDR1。我们的研究结果揭示了一种寡基因遗传结构，与之前报道的与ryanodine受体相关的单基因抗性形成对比。这项工作阐明了快速进化的抗药性的遗传基础，将有助于制定有效的抗药性管理策略。
