Steer K A, Sochor M, McLean P
Diabetes. 1985 May;34(5):485-90. doi: 10.2337/diab.34.5.485.
An examination was made of the effect of different periods of experimental diabetes on the activity of the pentose phosphate pathway in rat kidney. A rapid increase in kidney weight, expressed both in absolute terms and in terms of body weight, occurred shortly after the induction of diabetes. The activity of the enzymes of the oxidative segment of the pentose phosphate pathway and the flux of glucose through the pathway were both increased during the first 7 days after induction of diabetes. Thereafter, enzyme activity returned toward control levels, but the increased functional activity of the pathway, as measured using specifically labeled glucose, persisted. In contrast, transketolase was significantly depressed at the time of most rapid kidney growth. A positive correlation was found between the rate of kidney growth and the change in activity of glucose-6-phosphate dehydrogenase and a negative correlation with changes in transketolase activity. The possible roles of the oxidative and nonoxidative segments of the pentose phosphate pathway in the kidney in early diabetes-induced renal hypertrophy are discussed.
研究了实验性糖尿病不同时期对大鼠肾脏磷酸戊糖途径活性的影响。糖尿病诱导后不久，肾脏重量迅速增加，无论是以绝对值还是相对于体重而言。磷酸戊糖途径氧化部分的酶活性以及葡萄糖通过该途径的通量在糖尿病诱导后的前7天均增加。此后，酶活性恢复至对照水平，但使用特异性标记葡萄糖测量的该途径增加的功能活性持续存在。相比之下，在肾脏生长最快的时候转酮醇酶显著降低。发现肾脏生长速率与葡萄糖-6-磷酸脱氢酶活性变化呈正相关，与转酮醇酶活性变化呈负相关。讨论了磷酸戊糖途径的氧化和非氧化部分在早期糖尿病诱导的肾脏肥大中可能发挥的作用。
