晚间复合体组分ELF3招募H3K4me3去甲基化酶以抑制拟南芥中的光敏色素互作因子4和5。
The evening complex component ELF3 recruits H3K4me3 demethylases to repress PHYTOCHROME INTERACTING FACTOR4 and 5 in Arabidopsis.
作者信息
Tian Shiyu, Zhang Shen, Xu Fan, Sun Qingbin, Xu Gang, Ni Min
机构信息
State Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Taian 271018, China.
Department of Plant and Microbial Biology, University of Minnesota at Twin Cities, Saint Paul, MN 55108, USA.
出版信息
Plant Cell. 2024 Dec 23;37(1). doi: 10.1093/plcell/koaf014.
In Arabidopsis (Arabidopsis thaliana), light and circadian clock signaling converge on PHYTOCHROME-INTERACTING FACTORS (PIFs) 4 and 5 to produce a daily rhythm of hypocotyl elongation. PIF4 and PIF5 expression is repressed at dusk by the evening complex (EC), consisting of EARLY FLOWERING3 (ELF3), ELF4, and LUX ARRHYTHMO (LUX). Here, we report that ELF3 recruits the JUMONJI (JMJ) H3K4me3 demethylases JMJ17 and JMJ18 to the PIF4 and PIF5 loci in the evening to remove their H3K4me3 marks. The association of JMJ17 and JMJ18 with the 2 genomic loci depends on the EC, and the H3K4me3 marks are enriched in the elf3 and jmj17 jmj18 mutants. Half of the globally differentially expressed genes are overlapping in elf3 and jmj17 jmj18. Cleavage Under Targets and Tagmentation sequencing analysis identified 976 H3K4me3-enriched loci in elf3. Aligning the H3K4me3-enriched loci in elf3 to genes with increased expression in elf3 and jmj17 jmj18 identified 179 and 176 target loci, respectively. Half of the loci are targeted by both ELF3 and JMJ17/JMJ18. This suggests a strong connection between the 2 JMJ proteins and EC function. Our studies reveal that an array of key genes in addition to PIF4 and PIF5 are repressed by the EC through the H3K4me3 demethylation pathway.
在拟南芥（Arabidopsis thaliana）中，光信号和生物钟信号汇聚于与光敏色素相互作用因子（PIFs）4和5，以产生下胚轴伸长的昼夜节律。傍晚时，由早花3（ELF3）、ELF4和LUX节律失调蛋白（LUX）组成的傍晚复合体（EC）会抑制PIF4和PIF5的表达。在此，我们报告称，傍晚时ELF3会招募JUMONJI（JMJ）H3K4me3去甲基化酶JMJ17和JMJ18至PIF4和PIF5基因座，以去除它们的H3K4me3标记。JMJ17和JMJ18与这两个基因组位点的结合依赖于傍晚复合体，并且H3K4me3标记在elf3和jmj17 jmj18突变体中富集。在elf3和jmj17 jmj18中，全球差异表达基因的一半是重叠的。靶向切割和标签化测序分析在elf3中鉴定出976个H3K4me3富集位点。将elf3中H3K4me3富集位点与elf3和jmj17 jmj18中表达增加的基因进行比对，分别鉴定出179个和176个靶位点。一半的位点同时被ELF3和JMJ17/JMJ18靶向。这表明这两种JMJ蛋白与傍晚复合体功能之间存在紧密联系。我们的研究表明，除了PIF4和PIF5之外，一系列关键基因也通过H3K4me3去甲基化途径被傍晚复合体抑制。
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