Sun Yongjie, Zhang Dan, Dong Zhiqiang, Lyu Jinxiao, Wang Chunfei, Gong Jun, Wong Koon Ho, Wu Changfeng, Zhang Xuanjun
Faculty of Health Sciences, University of Macau, Taipa, Macau SAR, China.
MOE Frontiers Science Center for Precision Oncology, University of Macau, Taipa, Macau SAR, China.
Nat Commun. 2025 Jan 29;16(1):1115. doi: 10.1038/s41467-025-56253-7.
The fabrications of circularly polarized luminescent (CPL) material are mainly based on the chemical and physical strategies. Controlled biosynthesis of CPL-active materials is beset with difficulties due to the lack of bioactive luminescent precursors and bio-reactors. Enlighted by microbe-assisted asymmetric biosynthesis, herein, we show the in situ bacterial fermentation of Komagataeibacter sucrofermentants to fabricate a series of bacterial cellulosic biofilms with CPL of green, orange, red, and near-infrared colors. This process can trigger CPL emission for CPL-silent glycosylated luminophores and amplify the g of weak CPL-active luminophores up to a 10 scale. To confirm glycosidic bonds formation during the bacterial copolymerization process, we develop an assay utilizing the cellulase-catalyzed biodegradation of BC hybrids. More importantly, we achieve the information encryption and Fe dual-channel detection based on hybrid bacterial cellulosic biofilms. Therefore, this study not only provides another vision for CPL materials preparation but also broadens the application of bacterial cellulosic hybrids.
圆偏振发光（CPL）材料的制备主要基于化学和物理策略。由于缺乏生物活性发光前体和生物反应器，CPL活性材料的可控生物合成面临困难。受微生物辅助不对称生物合成的启发，在此我们展示了嗜蔗糖 Komagataeibacter 菌的原位细菌发酵，以制备一系列具有绿色、橙色、红色和近红外颜色CPL的细菌纤维素生物膜。该过程可以触发CPL沉默的糖基化发光体的CPL发射，并将弱CPL活性发光体的g值放大到10倍。为了确认细菌共聚过程中糖苷键的形成，我们开发了一种利用纤维素酶催化BC杂化物生物降解的测定方法。更重要的是，我们基于混合细菌纤维素生物膜实现了信息加密和铁的双通道检测。因此，本研究不仅为CPL材料的制备提供了另一种视角，也拓宽了细菌纤维素杂化物的应用范围。
