The role of defatted hydrolyzed egg yolk powder in protecting DHA algal oil.

This study comprehensively investigated DHA algal oil emulsions and microcapsules prepared with different egg yolk hydrolysates (DHYP). Dual-enzyme (phospholipase A1 and protease) treatment enhanced emulsion stability by boosting protein adsorption, reducing particle size, and increasing zeta potential. For microcapsules, EF-DUAL, treated with dual-enzymes, had improved solubility, dispersibility, and wall material compactness, effectively protecting DHA from oxidation. During accelerated storage, EF-DUAL had the lowest oxidation levels and maintained a high DHA retention rate of 22.08 % after 12 days at 60 °C, extending DHA algal oil's shelf life by 300 %. Linear regression analysis indicated that the oxidation of DHA algal oil followed first-order kinetics, whereas microcapsule powders exhibited higher zero-order coefficients. Overall, this study underscores the potential of DHYP, particularly dual-enzyme hydrolyzed egg yolk powder, as a wall material for DHA microencapsulation.