用于诊断慢性肉芽肿病的功能测定
Functional assays for the diagnosis of chronic granulomatous disease.
作者信息
Priel Debra Long, Lau Karen, Fink Danielle L, Kuhns Douglas B
机构信息
Clinical Services Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, United States of America.
Clinical Services Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, United States of America.
出版信息
J Immunol Methods. 2025 Feb;537:113820. doi: 10.1016/j.jim.2025.113820. Epub 2025 Jan 28.
Abstract
Chronic granulomatous disease (CGD) is a rare immunodeficiency characterized by recurrent bacterial and fungal infections that are attributed to reduced production of reactive oxygen species (ROS) by a multi-component enzyme complex known as the phagocyte NADPH oxidase or NOX2. Presented in this review are descriptions of several assays that assess the production of ROS as well as assays that characterize the expression of specific proteins of NOX2.
摘要
慢性肉芽肿病（CGD）是一种罕见的免疫缺陷病，其特征为反复发生细菌和真菌感染，这归因于一种称为吞噬细胞NADPH氧化酶或NOX2的多组分酶复合物产生活性氧（ROS）的能力降低。本综述介绍了几种评估ROS产生的检测方法以及表征NOX2特定蛋白表达的检测方法。
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