Vasuki Venkatesan, Krishnamoorthy Kaliannagounder, Subramanian Swaminathan, Sadanandane Candasamy, Balasubramaniyan Ramalingam, Sivagnaname Neelavathi, Padmanaban Veerappan, Vijayakumar Balakrishnan, Sundarraj Vinayagam, Palaniswamy Chokkalingam, Baral Prameela, Jambulingam Purushothaman
ICMR-Vector Control Research Centre, Puducherry, India.
ICMR-Vector Control Research Centre, Puducherry, India.
Int J Infect Dis. 2025 Mar;152:107807. doi: 10.1016/j.ijid.2025.107807. Epub 2025 Jan 28.
Molecular xenomonitoring (MX) is a recommended post-validation surveillance tool for detecting early signs of lymphatic filariasis (LF) transmission. This study reports the MX results for Culex quinquefasciatus transmitted Wuchereria bancrofti in three evaluation units (EUs) in different settings of LF elimination in India.
Female Cx. quinquefasciatus mosquitoes were collected using gravid traps from 150 households in each EU. Two pools of 25 mosquitoes from each household were assayed by real-time quantitative polymerase chain reaction for W. bancrofti DNA and RNA. The agreement between MX and the transmission assessment survey (TAS) in the mass drug administration (MDA) stopping decision was assessed. Additionally, the microfilaria (Mf) prevalence was compared in TAS-failed EU.
Vector infection prevalence was 0.05% and 0.07% in TAS-cleared EUs and 1.85% in TAS-failed EU. MX corroborated the TAS decision in all three settings. Mf prevalence was >1% at five sites in TAS-failed EU. Infective mosquitoes were detected in the TAS2 passed and TAS-failed EUs. The cost of MX per pool was between US $ 26.0 and 27.9 in different settings.
MX is a potential tool for evaluating different stages of post-MDA treatment in the LF elimination program. The MX detects transmission risk areas that can be confirmed by human surveys for appropriate response.
分子异体监测（MX）是一种推荐用于检测淋巴丝虫病（LF）传播早期迹象的验证后监测工具。本研究报告了在印度不同LF消除环境下的三个评估单位（EUs）中，致倦库蚊传播的班氏吴策线虫的MX结果。
在每个评估单位，使用诱蚊产卵器从150户家庭中收集雌性致倦库蚊。每个家庭的25只蚊子分成两组，通过实时定量聚合酶链反应检测班氏吴策线虫的DNA和RNA。评估了在大规模药物 administration（MDA）停药决策中MX与传播评估调查（TAS）之间的一致性。此外，比较了TAS评估失败的评估单位中的微丝蚴（Mf）流行率。
在TAS评估通过的评估单位中，媒介感染率分别为0.05%和0.07%，在TAS评估失败的评估单位中为1.85%。MX在所有三种情况下都证实了TAS的决策。在TAS评估失败的评估单位中的五个地点，Mf流行率>1%。在TAS2通过和TAS评估失败的评估单位中均检测到感染性蚊子。在不同环境下，每个样本池的MX成本在26.0美元至27.9美元之间。
MX是在LF消除计划中评估MDA后治疗不同阶段的潜在工具。MX检测到的传播风险区域可通过人体调查进行确认，以便做出适当反应。
