Do Thi Kieu Tiên, Trettin Ilona, Reich Eike
CAMAG, Muttenz, Switzerland.
HPTLC Association, Rheinfelden, Switzerland.
Methods Mol Biol. 2025;2895:47-61. doi: 10.1007/978-1-0716-4350-1_4.
High-performance thin-layer chromatography (HPTLC) plays a crucial role in establishing the chemical fingerprint of natural products (NPs) during analysis. This technique involves standardized procedures for each step, ensuring the reproducibility of results. In this context, we present a comprehensive standard operating procedure (SOP) encompassing both manual instruments and suitable devices. The methods detailed herein adopt a non-targeted screening approach applicable to all unrelated samples, complemented by a two-step derivatization process capable of detecting a wide range of phytochemical classes. By following these procedures, researchers can obtain reliable and consistent results that could be used to build a database for NPs using HPTLC.
高效薄层色谱法（HPTLC）在天然产物（NP）分析过程中确定其化学指纹图谱方面发挥着关键作用。该技术的每个步骤都涉及标准化程序，以确保结果的可重复性。在此背景下，我们提出了一个全面的标准操作规程（SOP），涵盖手动仪器和适用设备。本文详述的方法采用适用于所有不相关样品的非靶向筛选方法，并辅以能够检测多种植物化学类别的两步衍生化过程。通过遵循这些程序，研究人员可以获得可靠且一致的结果，这些结果可用于利用HPTLC建立NP数据库。
