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Flow cytometric measurements and electron microscopy of cell surface glycosaminoglycans using acridine orange.

作者信息

Timar J, Boldog F, Kopper L, Lapis K

出版信息

Histochem J. 1985 Jan;17(1):71-9. doi: 10.1007/BF01003404.

DOI:10.1007/BF01003404
PMID:3988548
Abstract

Cell surface glycosaminoglycans (GAGs) were measured, after various treatments, by their binding to Acridine Orange using flow cytometry. Using a critical electrolyte concentration and combining it with specific degradation of individual GAG elements, it was found possible to differentiate between GAG components. The technique was adapted for electron microscopy level to reveal characteristics of membrane-associated GAG. By this means, the cell membrane of the human leukaemic cell line K562 was shown to contain a large amount of GAG; 75% of it was highly sulphated GAG, mostly heparan sulphate. This component was evenly distributed in the outer plasma membrane layer. In the presence of other GAGs, the appearance of complex proteoglycan granules was detected.

摘要

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本文引用的文献

1
HISTOCHEMICAL IDENTIFICATION OF ACID MUCOPOLYSACCHARIDES WITH ACRIDINE ORANGE.用吖啶橙对酸性粘多糖进行组织化学鉴定
J Histochem Cytochem. 1964 Mar;12:164-70. doi: 10.1177/12.3.164.
2
Acridine orange stabilization of glycosaminoglycans in beginning endochondral ossification. A comparative light and electron microscopic study.吖啶橙对软骨内成骨起始阶段糖胺聚糖的稳定作用。一项光镜与电镜对比研究。
Histochemistry. 1981;70(2):107-14. doi: 10.1007/BF00493202.
3
Glycosaminoglycans in human cerebral tumors. Part II. Histochemical findings and correlations.
人类脑肿瘤中的糖胺聚糖。第二部分。组织化学研究结果及相关性。
Acta Neuropathol. 1982;57(4):299-305. doi: 10.1007/BF00692187.
4
Differentiated microdomains on the luminal surface of the capillary endothelium. II. Partial characterization of their anionic sites.毛细血管内皮细胞腔表面的分化微区。II. 其阴离子位点的部分特征
J Cell Biol. 1981 Sep;90(3):614-21. doi: 10.1083/jcb.90.3.614.
5
Characterization of heparan sulfate proteoglycans synthesized by rat ovarian granulosa cells in culture.培养的大鼠卵巢颗粒细胞合成的硫酸乙酰肝素蛋白聚糖的特性分析。
J Biol Chem. 1983 Nov 10;258(21):12857-64.
6
Histochemical evidence for lysosomal storage of acid glycosaminoglycans in splenic cells of rats treated with tilorone.用泰洛龙处理的大鼠脾细胞中酸性糖胺聚糖溶酶体储存的组织化学证据。
Histochemistry. 1982;76(1):71-87. doi: 10.1007/BF00493287.
7
Variability of cell surface glycoconjugates--relation to differences in cell function.
J Histochem Cytochem. 1981 Sep;29(9):994-1002. doi: 10.1177/29.9.6169762.
8
Heparan sulfate of AH-130 ascites hepatoma cells: a cell-surface glycosaminoglycan not displaced by heparin.AH - 130腹水肝癌细胞的硫酸乙酰肝素：一种不被肝素取代的细胞表面糖胺聚糖。
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