Ma Rulan, Nie Hongmei, Mo Caijing, Yuan Dawei, Zhu Kun, Li Kang
Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.
Department of Obstetrics and Gynecology, The Public Hospital of Feicheng, Feicheng, 271600, Shandong, China.
Eur J Med Res. 2025 Jan 30;30(1):59. doi: 10.1186/s40001-025-02290-3.
The effect of coiled-coil domain-containing 154 (CCDC154) in liver cancer (LC) remains unexplored. The objective of this study was to investigate the role of CCDC154 in LC and its underlying mechanism.
The analysis of CCDC154 expression and prognosis was performed using UALCAN, Human Protein Atlas and Kaplan-Meier plotter websites. Protein expression was measured using Western blotting assay. Lentivirus was used to silence CCDC154 expression in LC cells. The proliferation and apoptosis of LC cells was evaluated by cell counting assay, colony formation assay and flow cytometry. The migration and invasion of LC cells were investigated using scratch wound-healing assay and Transwell assay.
The results showed that CCDC154 was highly expressed in LC and related to tumor grade and stage. High CCDC154 expression was associated with to poor outcomes in LC patients. Silencing of CCDC154 inhibited proliferation, migration and invasion of LC cells. It also increased apoptosis in LC cells. After CCDC154 knockdown, the expression of Twist, Vimentin and Snail was down-regulated. Overexpression of Snail abated the inhibitory caused by CCDC154 knockdown on LC cell growth.
CCDC154 knockdown suppressed LC development through reducing Snail expression.
含卷曲螺旋结构域蛋白154（CCDC154）在肝癌（LC）中的作用尚未明确。本研究旨在探讨CCDC154在肝癌中的作用及其潜在机制。
利用UALCAN、人类蛋白质图谱和Kaplan-Meier绘图仪网站对CCDC154表达及预后进行分析。采用蛋白质印迹法检测蛋白表达。利用慢病毒沉默肝癌细胞中CCDC154的表达。通过细胞计数法、集落形成试验和流式细胞术评估肝癌细胞的增殖和凋亡。采用划痕愈合试验和Transwell试验研究肝癌细胞的迁移和侵袭能力。
结果显示，CCDC154在肝癌中高表达，且与肿瘤分级和分期相关。CCDC154高表达与肝癌患者不良预后相关。沉默CCDC154可抑制肝癌细胞的增殖、迁移和侵袭。它还增加了肝癌细胞的凋亡。敲低CCDC154后，Twist、波形蛋白和Snail的表达下调。过表达Snail可减轻CCDC154敲低对肝癌细胞生长的抑制作用。
敲低CCDC154通过降低Snail表达抑制肝癌发展。
