Zhang Si-Yu, Liu Zi-Teng, Zhao Xin-Di, Gao Zi-Yu, Jiang Ouyuan, Li Jie, Li Xiaoming, Kappler Andreas, Xu Jianming, Tang Xianjin
School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.
Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Environ Sci Technol. 2025 Feb 11;59(5):2541-2553. doi: 10.1021/acs.est.4c10809. Epub 2025 Jan 30.
Rice physiological straighthead disease is induced by microbially mediated arsenic methylation and usually regionally distributed in paddy soils. However, the biogeochemical mechanism underlying the geographic distribution of microbial communities harboring methylating genes () remains unclear. Herein, we revealed significant ( = 0.001) differences in the communities in different regions of Chinese paddy soils at the continental scale. Moreover, a positive correlation between the diversity of communities and the chemodiversity of soil dissolved organic matter (DOM) was revealed. Among the different DOM components, lignin- and peptide-like DOM are the most important DOM components impacting the abundance and diversity of communities. Metatranscriptomic analyses of 18 selected paddy soil samples revealed that the expression of the gene increased with increasing soil lignin and peptide contents. Compared with the control, the addition of lignin and peptide significantly ( < 0.05) increased the methylated As concentration in the incubated paddy soils. Communities harboring genes belonging to the phyla , , , , and mostly dominated in paddy soils with relatively high lignin and peptide contents. This study highlights the correlation between the diversity of DOM and communities in paddy soils and provides mechanistic information for soil arsenic contamination control and sustainable rice production.
水稻生理性直穗病由微生物介导的砷甲基化诱导，通常在稻田土壤中呈区域分布。然而，携带甲基化基因（）的微生物群落地理分布背后的生物地球化学机制仍不清楚。在此，我们揭示了在大陆尺度上中国稻田土壤不同区域的群落存在显著差异（=0.001）。此外，还揭示了群落多样性与土壤溶解有机物（DOM）化学多样性之间存在正相关关系。在不同的DOM组分中，木质素类和肽类DOM是影响群落丰度和多样性的最重要的DOM组分。对18个选定稻田土壤样品的宏转录组分析表明，基因的表达随着土壤木质素和肽含量的增加而增加。与对照相比，添加木质素和肽显著（<0.05）提高了培养的稻田土壤中甲基化砷的浓度。携带属于门、、、、的基因的群落在木质素和肽含量相对较高的稻田土壤中占主导地位。本研究突出了稻田土壤中DOM多样性与群落之间的相关性，并为土壤砷污染控制和水稻可持续生产提供了机制信息。
