Chen Long, Zhang Zhaoyuan, Li Niandong, Zhang Wanxia, Zheng Zhouhang, Zhang Yu
Guangzhou University of Chinese Medicine, Guangzhou, China.
Guangdong Provincial Second Hospital of Traditional Chinese Medicine, Guangzhou, China.
Front Vet Sci. 2025 Jan 16;11:1490769. doi: 10.3389/fvets.2024.1490769. eCollection 2024.
Animal models of LFH are still in the exploratory stage. This study aimed to establish a reliable, efficient, and economical model of LFH in rats for the study of human ligamentum flavum (LF) pathological mechanisms, drug screening, development, improvement of surgical treatment, disease prevention, and other aspects.
Forty rats were divided into an experimental group and a sham group of 20 rats. The experimental group ( = 20) was treated with an innovative operation combined with stress stimulation at the L5-L6 segments, the L5 and L6 spinous processes, transverse processes, and supraspinous ligaments were excised, along with removal of the paraspinal muscles at the L5-L6 level. One week after surgery, the rats were subjected to slow treadmill running daily. In the experimental group ( = 20), the spinous process, transverse process, supraspinous ligament and paraspinous muscle of L5 and L6 were excised. And for a week after the surgery, the rats ran on a treadmill at a slow pace every day. While the sham group ( = 20) was treated with sham operation only. Seven weeks later, MRI, immunohistochemistry (IHC), and western blot (WB) will be performed on the LF of the L5-6 segment in the two groups of rats.
MRI results showed that the LF in the experimental group was significantly thicker than that in the sham group. Masson staining results indicated that LF thickness, collagen fiber area, and collagen volume fraction (CVF) were significantly higher in the experimental group than in the sham group. IHC and WB showed that the expression of TGF-β1, COL1, and IL-1β in the LF of the experimental group was significantly higher than that in the LF of sham group.
Through innovative surgical intervention combined with stress stimulation, a relatively reliable, efficient, and convenient rat LFH model was established.
腰椎黄韧带肥厚（LFH）的动物模型仍处于探索阶段。本研究旨在建立一种可靠、高效且经济的大鼠LFH模型，用于研究人类黄韧带（LF）的病理机制、药物筛选、开发、改进手术治疗、疾病预防等方面。
40只大鼠分为实验组和假手术组，每组20只。实验组（n = 20）采用创新手术联合L5 - L6节段的应激刺激，切除L5和L6棘突、横突及棘上韧带，并去除L5 - L6水平的椎旁肌。术后1周，大鼠每天进行慢速跑步机跑步。实验组（n = 20）切除L5和L6的棘突、横突、棘上韧带及椎旁肌。术后1周内，大鼠每天在跑步机上缓慢跑步。而假手术组（n = 20）仅进行假手术。7周后，对两组大鼠L5 - 6节段的LF进行磁共振成像（MRI）、免疫组织化学（IHC）和蛋白质免疫印迹（WB）检测。
MRI结果显示，实验组的LF明显比假手术组厚。Masson染色结果表明，实验组的LF厚度、胶原纤维面积和胶原体积分数（CVF）均显著高于假手术组。IHC和WB显示，实验组LF中转化生长因子-β1（TGF-β1）、Ⅰ型胶原（COL1）和白细胞介素-1β（IL-1β）的表达明显高于假手术组LF。
通过创新的手术干预联合应激刺激，建立了一种相对可靠、高效且便捷的大鼠LFH模型。
