Könker Jana, Zenker Sanja, Meierhenrich Anja, Patel Anant, Dietz Karl-Josef
Plant Biochemistry and Physiology, Bielefeld University, Bielefeld, Germany.
Fermentation and Formulation of Biologicals and Chemicals, Hochschule Bielefeld - University of Applied Sciences and Arts, Bielefeld, Germany.
Front Plant Sci. 2025 Jan 16;15:1497575. doi: 10.3389/fpls.2024.1497575. eCollection 2024.
The network of antagonistic, neutral, and synergistic interactions between (micro)organisms has moved into the focus of current research, since in agriculture, this knowledge can help to develop efficient biocontrol strategies. Applying the nematophagous fungus as biocontrol agent to manage the root-knot nematode is a highly promising strategy. To gain new insight into the systemic response of plants to a plant-parasitic nematode and a nematophagous fungus, was inoculated with and/or and subjected to transcriptome and metabolome analysis of leaves. While the metabolome proved quite stable except for the early time point of 48 h, comparison of the single with the combined treatment revealed even larger effects after 6 d compared to 48 h, aligning with the later root infestation by compared to . Simultaneous exposure to both microorganisms showed a stronger overlap with the single treatment than . Changes of transcripts and metabolites were higher in the combined treatment compared to the individual inoculations. The results support the conclusion that induces plant defense in a distinct and beneficial manner if combined with although plant defense is partly suppressed by the endophytic growth. The results tentatively suggested that the application of as a biocontrol agent against can be more effective by supporting these tritrophic interactions with specific additives, such as phytohormones or amino acids in the formulation.
（微生物之间）拮抗、中性和协同相互作用的网络已成为当前研究的重点，因为在农业中，这方面的知识有助于制定高效的生物防治策略。应用食线虫真菌作为生物防治剂来防治根结线虫是一种非常有前景的策略。为了深入了解植物对植物寄生线虫和食线虫真菌的系统反应，对植物接种线虫和/或真菌，并对叶片进行转录组和代谢组分析。除了48小时这个早期时间点外，代谢组表现得相当稳定，与单一处理相比，联合处理在6天后显示出比48小时更大的效应，这与线虫比真菌更晚侵染根部相一致。同时暴露于两种微生物下，与单一真菌处理相比，显示出与单一线虫处理有更强的重叠。与单独接种相比，联合处理中转录本和代谢物的变化更大。结果支持了这样的结论：如果与线虫联合使用，真菌会以一种独特且有益的方式诱导植物防御，尽管植物防御会被内生生长部分抑制。结果初步表明，通过在制剂中添加特定添加剂（如植物激素或氨基酸）来支持这些三营养相互作用，应用真菌作为防治线虫的生物防治剂可能会更有效。
