Baidya Shouvik, Biswas Nipa, Chowdhury Bratati, Chakraborty Binayak, Kumar Deepak, Karmakar Kapudeep
Department of Soil Science and Agricultural Chemistry, Uttar Banga Krishi Viswavidayalaya, Cooch Behar, India.
Department of Seed Science and Technology, Uttar Banga Krishi Viswavidayalaya, Cooch Behar, India.
J Sci Food Agric. 2025 Mar 30;105(5):3133-3138. doi: 10.1002/jsfa.14153. Epub 2025 Jan 31.
Low soil temperature and its fluctuation can negatively impact the growth of seedlings. The district of Cooch Behar (India), belonging to the Cwa zone (according to Koppen's classification), receives several cold waves during winter. Our previous study demonstrated that a constant temperature of 20 °C (chilling but not freezing) can cause a loss in the vigor of tomatoes. Since the temperature of the soil is not uniform throughout the day, we hypothesized that the duration of cold exposure can have variable effects on seed vigor.
It was observed that increasing the duration of cold stress can slow down the germination process and reduce vigor. This was due to the cold-mediated damage to cell membranes (due to dehydration) which caused electrolyte leakage and reduced levels of glutathione reductase. In this regard, biopriming seeds with microbes that produce exopolysaccharide (EPS) can be useful as it can form a protective layer on the seeds. Indigenous EPS-producing bacteria, Bacillus, Phytobacter and Priestia sp., were used for biopriming. Priestia and Phytobacter sp. not only reduced the electrolyte leakage but also increased the levels of antioxidant genes. This improved the germination speed and vigor. In a field trial, the rhizosphere of the seedlings pretreated with bioinoculants displayed a reduced thermal fluctuation compared with the untreated seeds.
The seedlings treated with bioinoculants grew faster in soil in spite of low soil temperature. This can reduce the nursery time of seedlings. © 2025 Society of Chemical Industry.
土壤低温及其波动会对幼苗生长产生负面影响。印度科奇比哈尔地区属于Cwa气候区（根据柯本气候分类法），冬季会遭受多次寒潮侵袭。我们之前的研究表明，20°C的恒温（低温但不结冰）会导致番茄活力下降。由于土壤温度全天并不均匀，我们推测冷暴露的持续时间可能对种子活力产生不同影响。
观察到冷胁迫持续时间增加会减缓发芽过程并降低活力。这是由于冷介导的细胞膜损伤（由于脱水）导致电解质渗漏和谷胱甘肽还原酶水平降低。在这方面，用产生胞外多糖（EPS）的微生物对种子进行生物引发可能会有帮助，因为它可以在种子上形成保护层。使用本地产生EPS的细菌芽孢杆菌属、植物杆菌属和Priestia菌属进行生物引发。Priestia菌属和植物杆菌属不仅减少了电解质渗漏，还提高了抗氧化基因的水平。这提高了发芽速度和活力。在田间试验中，与未处理的种子相比，用生物菌剂预处理的幼苗根际温度波动较小。
尽管土壤温度较低，但用生物菌剂处理的幼苗在土壤中生长更快。这可以减少幼苗的育苗时间。©2025化学工业协会。
