• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

线粒体蛋白MjEF-Tu由引发免疫信号和抗性的线虫分泌到宿主植物中。

Mitochondrial Protein MjEF-Tu is Secreted into Host Plants by Nematodes Eliciting Immune Signaling and Resistance.

作者信息

Lin Borong, Huang Shaozhen, Li Zhiwen, Huang Qiuling, Song Handa, Fang Tianyi, Liao Jinling, Gheysen Godelieve, Zhuo Kan

机构信息

College of Plant Protection, South China Agricultural University, Guangzhou, 510642, China.

Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, China.

出版信息

Adv Sci (Weinh). 2025 Mar;12(11):e2412968. doi: 10.1002/advs.202412968. Epub 2025 Jan 30.

DOI:10.1002/advs.202412968
PMID:39888272
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11923865/
Abstract

Little is known about plant-parasitic animal-derived pathogen-associated molecular pattern (PAMP)/ pattern-recognition receptor (PRR) pairs. Additionally, mitochondrial proteins have not previously been reported to be secreted into hosts by pathogens. Here, it is found that the Meloidogyne javanica elongation factor thermo unstable (EF-Tu) (MjEF-Tu) located in the nematode mitochondria is up-regulated and secreted into the host plant during nematode parasitism. MjEF-Tu interacts with the PRR Arabidopsis thaliana EF-Tu receptor (AtEFR), triggering the plant hallmark defence responses mediated by AtEFR. An 18-aa sequence (Nelf18) in the N terminus of the nematode EF-Tu contributes to the immunogenic activity. M. javanica water extract and mitochondrial extract also induce plant immunity sensed by AtEFR, owing to the presence of MjEF-Tu. In addition, Nelf18 enhances plant resistance to nematode, virus, and bacterial infections depending on AtEFR. These findings first demonstrate that mitochondrial proteins from pathogens can be secreted into hosts and function as a cross-kingdom signal and identified the first plant-parasitic animal-derived proteinaceous PAMP/PRR pair, providing novel insights into host-pathogen interactions.

摘要

关于植物寄生性动物源病原体相关分子模式（PAMP）/模式识别受体（PRR）对，人们了解甚少。此外，此前尚无线粒体蛋白被病原体分泌到宿主中的报道。在此，研究发现，位于线虫线粒体中的爪哇根结线虫延伸因子热不稳定蛋白（MjEF-Tu）在寄生过程中表达上调并分泌到宿主植物中。MjEF-Tu与PRR拟南芥EF-Tu受体（AtEFR）相互作用，触发由AtEFR介导的植物标志性防御反应。线虫EF-Tu N端的一个18个氨基酸的序列（Nelf18）有助于免疫原活性。由于MjEF-Tu的存在，爪哇根结线虫水提取物和线粒体提取物也能诱导AtEFR感知的植物免疫。此外，Nelf18依赖AtEFR增强植物对线虫、病毒和细菌感染的抗性。这些发现首次证明病原体的线粒体蛋白可分泌到宿主中并作为一种跨界信号发挥作用，同时鉴定出首个植物寄生性动物源蛋白质类PAMP/PRR对，为宿主-病原体相互作用提供了新见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/b1f90f20b94a/ADVS-12-2412968-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/c7bf95645adf/ADVS-12-2412968-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/badcc2aaa6c3/ADVS-12-2412968-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/327076a73521/ADVS-12-2412968-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/8862280ce10e/ADVS-12-2412968-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/b7df08f800b1/ADVS-12-2412968-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/b1f90f20b94a/ADVS-12-2412968-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/c7bf95645adf/ADVS-12-2412968-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/badcc2aaa6c3/ADVS-12-2412968-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/327076a73521/ADVS-12-2412968-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/8862280ce10e/ADVS-12-2412968-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/b7df08f800b1/ADVS-12-2412968-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdd5/11923865/b1f90f20b94a/ADVS-12-2412968-g003.jpg

相似文献

1
Mitochondrial Protein MjEF-Tu is Secreted into Host Plants by Nematodes Eliciting Immune Signaling and Resistance.线粒体蛋白MjEF-Tu由引发免疫信号和抗性的线虫分泌到宿主植物中。
Adv Sci (Weinh). 2025 Mar;12(11):e2412968. doi: 10.1002/advs.202412968. Epub 2025 Jan 30.
2
Arabidopsis EF-Tu receptor enhances bacterial disease resistance in transgenic wheat.拟南芥延伸因子Tu受体增强转基因小麦的细菌病害抗性。
New Phytol. 2015 Apr;206(2):606-13. doi: 10.1111/nph.13356. Epub 2015 Mar 11.
3
A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system.一种新型线虫效应蛋白通过激活宿主活性氧清除系统来抑制植物免疫。
New Phytol. 2016 Feb;209(3):1159-73. doi: 10.1111/nph.13701. Epub 2015 Oct 20.
4
Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR.通过转移双子叶植物延伸因子 Tu 受体 EFR 增强单子叶植物水稻的固有免疫系统。
J Integr Plant Biol. 2015 Jul;57(7):641-52. doi: 10.1111/jipb.12306. Epub 2015 Jan 13.
5
The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism.新型 30C02 胞囊线虫效应蛋白与植物 β-1,3-内切葡聚糖酶的相互作用可能抑制宿主防御以促进寄生。
J Exp Bot. 2012 Jun;63(10):3683-95. doi: 10.1093/jxb/ers058. Epub 2012 Mar 21.
6
Two distinct EF-Tu epitopes induce immune responses in rice and Arabidopsis.两个不同的 EF-Tu 表位在水稻和拟南芥中诱导免疫反应。
Mol Plant Microbe Interact. 2014 Feb;27(2):113-24. doi: 10.1094/MPMI-10-13-0304-R.
7
NRPS-like ATRR in Plant-Parasitic Nematodes Involved in Glycine Betaine Metabolism to Promote Parasitism.植物寄生线虫中参与甘氨酸甜菜碱代谢以促进寄生的类非核糖体肽合成酶（NRPS）ATRR
Int J Mol Sci. 2024 Apr 12;25(8):4275. doi: 10.3390/ijms25084275.
8
Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors.孢囊线虫的质外体毒液类过敏原蛋白通过细胞表面受体调节植物基础先天免疫的激活。
PLoS Pathog. 2014 Dec 11;10(12):e1004569. doi: 10.1371/journal.ppat.1004569. eCollection 2014 Dec.
9
The plant apoplasm is an important recipient compartment for nematode secreted proteins.植物质外体是线虫分泌蛋白的重要接受隔室。
J Exp Bot. 2011 Jan;62(3):1241-53. doi: 10.1093/jxb/erq352. Epub 2010 Nov 29.
10
Arabidopsis leucine-rich repeat receptor-like kinase NILR1 is required for induction of innate immunity to parasitic nematodes.拟南芥富含亮氨酸重复序列的类受体激酶NILR1是诱导对寄生线虫的先天免疫所必需的。
PLoS Pathog. 2017 Apr 13;13(4):e1006284. doi: 10.1371/journal.ppat.1006284. eCollection 2017 Apr.

本文引用的文献

1
NILR1 perceives a nematode ascaroside triggering immune signaling and resistance.NILR1感知一种线虫脂肪酸结合蛋白触发免疫信号传导和抗性。
Curr Biol. 2023 Sep 25;33(18):3992-3997.e3. doi: 10.1016/j.cub.2023.08.017. Epub 2023 Aug 28.
2
InterPro in 2022.InterPro 在 2022 年。
Nucleic Acids Res. 2023 Jan 6;51(D1):D418-D427. doi: 10.1093/nar/gkac993.
3
Isolation, profiling, and tracking of extracellular vesicle cargo in Caenorhabditis elegans.在秀丽隐杆线虫中细胞外囊泡货物的分离、分析和追踪。
Curr Biol. 2022 May 9;32(9):1924-1936.e6. doi: 10.1016/j.cub.2022.03.005. Epub 2022 Mar 24.
4
The N-terminus of an Ustilaginoidea virens Ser-Thr-rich glycosylphosphatidylinositol-anchored protein elicits plant immunity as a MAMP.玉米黑粉菌丝氨酸-苏氨酸丰富的糖基磷脂酰肌醇锚定蛋白 N 端作为一种模式分子激发植物免疫。
Nat Commun. 2021 Apr 27;12(1):2451. doi: 10.1038/s41467-021-22660-9.
5
Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.选择性地将线粒体蛋白包装到细胞外囊泡中可以防止线粒体 DAMPs 的释放。
Nat Commun. 2021 Mar 30;12(1):1971. doi: 10.1038/s41467-021-21984-w.
6
Protective plant immune responses are elicited by bacterial outer membrane vesicles.植物的保护性免疫反应是由细菌外膜囊泡引发的。
Cell Rep. 2021 Jan 19;34(3):108645. doi: 10.1016/j.celrep.2020.108645.
7
The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis.ImageJ 生态系统：用于图像可视化、处理和分析的开源软件。
Protein Sci. 2021 Jan;30(1):234-249. doi: 10.1002/pro.3993. Epub 2020 Nov 20.
8
The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis.延伸因子Tu（EF-Tu）在微生物致病机制中的多种功能作用
Front Microbiol. 2019 Oct 24;10:2351. doi: 10.3389/fmicb.2019.02351. eCollection 2019.
9
Detecting sequence signals in targeting peptides using deep learning.利用深度学习检测靶向肽中的序列信号。
Life Sci Alliance. 2019 Sep 30;2(5). doi: 10.26508/lsa.201900429. Print 2019 Oct.
10
Microparticles in the blood of patients with SLE: Size, content of mitochondria and role in circulating immune complexes.SLE 患者血液中的微粒体：大小、线粒体含量及其在循环免疫复合物中的作用。
J Autoimmun. 2019 Aug;102:142-149. doi: 10.1016/j.jaut.2019.05.003. Epub 2019 May 16.