Maisano F, Belew M, Porath J
J Chromatogr. 1985 Mar 15;321(2):305-17. doi: 10.1016/s0021-9673(01)90448-0.
A homologous series of uncharged thioalkyl derivatives of agarose were prepared by a simplified synthetic route and their adsorption behaviour towards human serum proteins was evaluated and compared with that of a commercially available alkyl ether derivative of agarose. The influence of the spacer arm length on the adsorption efficiency was also investigated. The degree of substitution of the derivatives can be estimated conveniently by sulphur analysis. The four different types of thiolkyl derivatives (C6, C8, C12 and C14) investigated here behave in all respects like hydrophobic adsorbents. The coupling yield obtained is high (75% or more) and is better than that obtained by alternative synthetic routes reported so far. The adsorption capacity towards serum proteins of the various derivatives increases with increasing alkyl chain length and degree of substitution. Desorption is achieved by a progressive decrease in the polarity of the eluent and the recovery of the applied material is in the range 80-90%. The role played by the thioether as a possible modulator of the observed hydrophobic adsorption is discussed. For the group separation of serum proteins the optimum adsorbent, as regards capacity combined with ease of elution of adsorbed material, should be substituted with chains of six or eight carbon atoms and have a ligand concentration in the range 80-120 mumole g-1 dry gel.
通过简化的合成路线制备了一系列不带电荷的琼脂糖硫代烷基衍生物，并评估了它们对人血清蛋白的吸附行为，并与市售的琼脂糖烷基醚衍生物进行了比较。还研究了间隔臂长度对吸附效率的影响。衍生物的取代度可通过硫分析方便地估算。本文研究的四种不同类型的硫代烷基衍生物（C6、C8、C12和C14）在各方面的表现都类似于疏水吸附剂。获得的偶联产率很高（75%或更高），优于迄今为止报道的其他合成路线。各种衍生物对血清蛋白的吸附容量随烷基链长度和取代度的增加而增加。通过逐步降低洗脱剂的极性实现解吸，所加物质的回收率在80-90%范围内。讨论了硫醚作为观察到的疏水吸附的可能调节剂所起的作用。对于血清蛋白的分组分离，就吸附容量和吸附物质洗脱的难易程度而言，最佳吸附剂应以含有六个或八个碳原子的链进行取代，且配体浓度在80-120微摩尔/克干凝胶范围内。
