Ikarashi Y, Sasahara T, Maruyama Y
J Chromatogr. 1985 Mar 29;322(1):191-9. doi: 10.1016/s0021-9673(01)97671-x.
Regional choline (Ch) and acetylcholine (ACh) in rat brain were clearly determined by high-performance liquid chromatography with electrochemical detection. The method is based on that of Potter et al.: the hydrogen peroxide that is enzymatically produced from both compounds is measured and a successful improvement of the method, particularly for purification, is described. Recoveries were 96.1 +/- 1.4% for Ch and 95.6 +/- 2.2% for ACh and amounts as low as 10 pmol could be determined. Prior to measuring the compounds, a newly developed magnetic field microwave instrument (10 kW) was utilized for the rapid inactivation of brain enzymes. The levels of Ch and ACh in brain regions were compared with those reported elsewhere.
采用高效液相色谱-电化学检测法可清晰测定大鼠脑中局部胆碱（Ch）和乙酰胆碱（ACh）的含量。该方法基于波特等人的方法：通过测量由这两种化合物酶促产生的过氧化氢，并描述了该方法的成功改进，特别是在纯化方面。Ch的回收率为96.1±1.4%，ACh的回收率为95.6±2.2%，最低可测定量为10皮摩尔。在测量这些化合物之前，使用了新开发的磁场微波仪器（10千瓦）来快速使脑酶失活。将脑区中Ch和ACh的水平与其他地方报道的水平进行了比较。
