采用高效液相色谱-质谱联用技术对未衍生化唾液酸进行结构分析。

Structural analysis of underivatized sialic acids by combined high-performance liquid chromatography-mass spectrometry.

作者信息

Shukla A K, Schauer R, Schade U, Moll H, Rietschel E T

出版信息

J Chromatogr. 1985 Feb 8;337(2):231-8. doi: 10.1016/0378-4347(85)80036-0.

Abstract

Mass spectra of chemically ionized, positive ions of underivatized N,O-acylated sialic acids, 2-deoxy-2,3-didehydro-N-acetylneuraminic acid and sialyl-alpha(2-3)-lactose were obtained by combined high-performance liquid chromatography--mass spectrometry, using a direct liquid inlet system. The mass spectra of the different compounds for which fragmentation schemes are proposed enable the differentiation between sialic acids, although the localization of O-substituents is not possible. However, since the various sialic acids separated well on high-performance liquid chromatography, combined high-performance liquid chromatography-mass spectrometry allowed their unequivocal characterization.

摘要

使用直接液体进样系统，通过高效液相色谱 - 质谱联用技术获得了未衍生化的N,O - 酰化唾液酸、2 - 脱氧 - 2,3 - 二脱氢 - N - 乙酰神经氨酸和唾液酸α(2 - 3) - 乳糖的化学电离正离子质谱图。尽管无法确定O - 取代基的位置，但所提出的不同化合物的碎裂模式的质谱图能够区分唾液酸。然而，由于各种唾液酸在高效液相色谱上分离良好，高效液相色谱 - 质谱联用技术能够对它们进行明确的表征。