Antioxidant activities of three enzymatic hydrolysates from on HaCaT cells and zebrafish .

To explore the antioxidant activity of enzymatic hydrolysates of from Dalian and preliminarily elucidate their mechanisms of action both and . Samples were hydrolysed using alcalase, protamex, and neutrase. 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays showed that the alcalase hydrolysate had the highest antioxidant activity, with IC values of 4.233 ± 0.067 mg/mL (DPPH) and 1.188 ± 0.066 mg/mL (ABTS). Further cell experiments indicated that the alcalase hydrolysate effectively inhibited intracellular reactive oxygen species (ROS), with a fluorescence rate of 4.29% ± 1.98%, and modulated antioxidant-related enzymes (superoxide dismutase: SOD, glutathione peroxidase: GSH-PX, malondialdehyde: MDA). The obtained optimal enzymatic hydrolysate was analysed by LC-MS/MS. The fluorescence rate of zebrafish (AAPH: 100%) decreased to 29.24% ± 1.10% (acridine orange: AO) and 56.71% ± 0.02% (ROS), with no observed embryonic toxicity. This study provides a foundation for the development of natural antioxidants.