Fu Peipei, Zhai Jiaying, Yang Xiaoao, Gao Jiaxin, Ren Zhongjie, Guo Baoying, Qi Pengzhi
National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang 316004, PR China.
National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang 316004, PR China.
J Hazard Mater. 2025 May 5;488:137399. doi: 10.1016/j.jhazmat.2025.137399. Epub 2025 Jan 27.
Antibiotic resistance genes (ARGs) raise a global public health concern. The ARGs profile in marine aquaculture environments was well reported, yet it is poorly revealed in marine bivalves. This study investigated the microbiota, resistome, and environmental factors within the digestive glands of two mussel species (Mytilus coruscus and Mytilus galloprovincialis) cultivated in the East China Sea and Yellow Sea. The microbial communities in the digestive glands of mussels exhibit significant variations across different sampling sites and between the two seas. The three bacterial phyla that predominated in all samples were Firmicutes, Bacteroidota, and Proteobacteria. A total of 88 ARGs were detected, with aminoglycoside resistance genes and multidrug resistance genes being the dominant categories. Analysis revealed that the quinolone resistance gene qnrB, associated with clinically relevant human pathogens, was ubiquitous in all samples. Members of the Enterobacteriaceae family may serve as a reservoir for qnrB within the investigated environment. The distribution of ARGs shows potential associations with the composition of microbial communities in the digestive glands, environmental factors, and mobile genetic elements (MGEs). These findings enhance the elucidation of microbial ecology and antibiotic resistance in marine aquaculture.
抗生素抗性基因（ARGs）引发了全球公共卫生关注。海洋水产养殖环境中的ARGs概况已有大量报道，但在海洋双壳贝类中却鲜有揭示。本研究调查了在中国东海和黄海养殖的两种贻贝（波纹巴非蛤和地中海贻贝）消化腺内的微生物群、抗性组和环境因素。贻贝消化腺中的微生物群落因不同采样地点以及两海之间的差异而表现出显著变化。所有样本中占主导地位的三个细菌门是厚壁菌门、拟杆菌门和变形菌门。共检测到88种ARGs，其中氨基糖苷类抗性基因和多药抗性基因是主要类别。分析表明，与临床相关人类病原体相关的喹诺酮抗性基因qnrB在所有样本中普遍存在。肠杆菌科成员可能是所研究环境中qnrB的储存库。ARGs的分布显示出与消化腺中微生物群落组成、环境因素和移动遗传元件（MGEs）之间存在潜在关联。这些发现增进了对海洋水产养殖中微生物生态学和抗生素抗性的理解。
