Peters A, Kara D A
J Comp Neurol. 1985 Apr 8;234(2):242-63. doi: 10.1002/cne.902340209.
In the preceding article the characteristics of the various types of pyramidal cells present in area 17 of rat visual cortex were described (Peters and Kara, '85). In the present article the nonpyramidal cell population of this cortex is considered. It is known from Golgi preparations that in layers II-VIa there are bipolar cells, smooth or sparsely spinous multipolar and bitufted cells with either unmyelinated local plexus or myelinated axons, and chandelier cells. Each of these cell types has been previously examined in Golgi-electron microscopic preparations. The question now being asked is whether the information about the characteristics of these different types of nonpyramidal cells derived from the Golgi-electron microscopic studies can be used to identify the cell bodies of nonpyramidal cells in tissue prepared for conventional electron microscopy. If this can be done then the neuronal composition of area 17 can be determined. It has been found that the cell bodies of bipolar cells can be readily identified because they are elongate and have nuclei with a vertical infolding and few axosomatic synapses, which are of both the symmetric and asymmetric varieties. Evidence is presented to show that there are two types of bipolar cells, small ones and large ones, the large ones being distinguished by their well-developed endoplasmic reticulum in which the cisternae are arranged parallel to the cell surface. Bipolar cells account for 6% of the neuronal profiles in layer II/III, 3% in layer IV, 5% in layer V, and 2% in layer VIa. The cell bodies of other types of nonpyramidal cells in layers II-VIa cannot be distinguished from each other in thin sections, because recognition of the different cell types depends upon the characteristics and distribution of their dendrites and axons. However, it is evident that in this group of neurons there are some with small cell bodies and others with large cell bodies, and in both size groups there are varieties of neurons which can be recognized from the characteristics of their perikaryal cytoplasm. All of these neurons have both symmetric and asymmetric axosomatic synapses. The greatest number of these nonpyramidal cells which are not bipolar in form is found within layer II/III, where they account for 7% of all neuronal profiles. These neurons comprise 4% of all neuronal profiles in layer IV, 6% in layer V, and 2% in layer VIa. Layers I and VIb contain only nonpyramidal cells, but these are different from the ones in layers II-VIa.(ABSTRACT TRUNCATED AT 400 WORDS)
在前一篇文章中描述了大鼠视皮层17区存在的各种类型锥体细胞的特征（彼得斯和卡拉，1985年）。在本文中，将探讨该皮层中的非锥体细胞群体。从高尔基染色标本可知，在第II - VIa层中有双极细胞、表面光滑或有稀疏棘突的多极细胞和双簇状细胞，它们有的具有无髓鞘的局部神经丛，有的具有有髓鞘的轴突，还有吊灯细胞。之前已在高尔基 - 电子显微镜标本中对这些细胞类型中的每一种进行了研究。现在提出的问题是，从高尔基 - 电子显微镜研究中获得的关于这些不同类型非锥体细胞特征的信息，是否可用于在为常规电子显微镜制备的组织中识别非锥体细胞的胞体。如果可以做到这一点，那么17区的神经元组成就可以确定。已发现双极细胞的胞体很容易识别，因为它们呈细长形，细胞核有垂直褶皱，且轴体突触较少，包括对称和不对称两种类型。有证据表明存在两种双极细胞，即小双极细胞和大双极细胞，大双极细胞的特征是其内质网发达，其中的潴泡与细胞表面平行排列。双极细胞在第II/III层的神经元轮廓中占6%，在第IV层占3%，在第V层占5%，在第VIa层占2%。在第II - VIa层中，其他类型非锥体细胞的胞体在薄切片中彼此无法区分，因为识别不同细胞类型取决于它们的树突和轴突的特征及分布。然而，很明显在这组神经元中，有些胞体小，有些胞体大，并且在这两个大小组中都有根据其核周细胞质特征可识别的多种神经元。所有这些神经元都有对称和不对称的轴体突触。这些非双极形态的非锥体细胞数量最多的是在第II/III层，它们占该层所有神经元轮廓的7%。这些神经元在第IV层占所有神经元轮廓的4%，在第V层占6%，在第VIa层占2%。第I层和第VIb层仅包含非锥体细胞，但它们与第II - VIa层中的细胞不同。（摘要截取自400字）
