Duan Geyan, Li Mengmeng, Zheng Changbing, Wan Mengliao, Yu Jiayi, Cao Bo, Yin Yulong, Duan Yehui, Cong Fang
Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China; University of Chinese Academy of Sciences, Beijing, China.
Wilmar (Shanghai) Biotechnology R&D Center Co., Ltd., Shanghai, China.
J Nutr. 2025 May;155(5):1298-1310. doi: 10.1016/j.tjnut.2025.01.021. Epub 2025 Jan 29.
The intestinal development and nutritional needs of piglets after birth are similar to those of human infants.
This study aimed to investigate the effect of different forms of odd-chain fatty acids (OCFAs) on the growth and intestinal morphology and function of milk replacer-fed piglets, as a model for human infants.
Forty 7-d-old piglets from 8 sows were randomly assigned into 5 groups (n = 8, each from a different litter) and fed sow milk or milk replacers supplemented with different kinds of fats (control fats, docosahexaenoic acid algal oil-, OCFA algal oil-, and OCFA-enriched fats) for 21 d. One-way analysis of variance was performed to compare the milk replacer-fed piglet groups, and unpaired t test was used to compare sow milk- and milk replacer-fed piglets.
Milk replacers supplemented with OCFA-enriched fats increased the average daily gain (ADG), ratio of villus height to crypt depth, and protein expression of Ki67, phosphorylated (p)-mTOR, p-S6K1, Occludin, Claudin, and ZO-1 in the selected intestines of piglets and decreased the protein expression of p-ULK1, Parkin, and PINK1 to levels similar to those of the sow milk-fed group (P < 0.05).
Overall, milk replacers supplemented with OCFA-enriched fats improved the ADG and the intestinal morphology and function of piglets to levels comparable to those of the sow milk-fed piglets.
仔猪出生后的肠道发育和营养需求与人类婴儿相似。
本研究旨在调查不同形式的奇数链脂肪酸（OCFAs）对以代乳粉喂养的仔猪生长、肠道形态和功能的影响，以此作为人类婴儿的模型。
将来自8头母猪的40只7日龄仔猪随机分为5组（每组8只，来自不同窝），分别用母乳或添加不同种类脂肪（对照脂肪、二十二碳六烯酸藻油、OCFA藻油和富含OCFA的脂肪）的代乳粉喂养21天。采用单因素方差分析比较代乳粉喂养的仔猪组，采用非配对t检验比较母乳和代乳粉喂养的仔猪。
添加富含OCFA脂肪的代乳粉提高了仔猪选定肠道的平均日增重（ADG）、绒毛高度与隐窝深度比值以及Ki67、磷酸化（p）-mTOR、p-S6K1、闭合蛋白、紧密连接蛋白和ZO-1的蛋白表达，并将p-ULK1、帕金蛋白和粉色荧光蛋白1的蛋白表达降低至与母乳喂养组相似的水平（P<0.05）。
总体而言，添加富含OCFA脂肪的代乳粉将仔猪的ADG以及肠道形态和功能改善至与母乳喂养仔猪相当的水平。
