Rumyantseva Natalia A, Shutov Vladimir M, Belenkaia Dina G, Alekseev Aleksandr A, Morozova Natalia E, Vedyaykin Alexey D
Peter the Great St.Petersburg Polytechnic University, Institute of Biomedical Systems and Biotechnology, Polytechnicheskaya 29,Saint Petersburg 195251, Russia.
FEMS Microbiol Lett. 2025 Jan 10;372. doi: 10.1093/femsle/fnaf010.
SMC (Structural Maintenance of Chromosomes) ATPase proteins are integral components of complexes bearing the same name, crucial for the spatial organization of DNA across diverse life forms, spanning bacteria, archaea, and eukaryotes. It is proposed that in bacteria, SMC complexes facilitate DNA compaction through loop extrusion and aid in the segregation of daughter nucleoids. In this paper, the properties of the SMC ATPase protein from Ureaplasma parvum were investigated by using a spectrum of methods, including conventional biochemical methods as well as advanced single-molecule techniques. Our findings reveal distinctive properties of this protein compared to its extensively studied homologue from Bacillus subtilis. Notably, our results suggest that U. parvum Smc ATPase facilitates DNA compaction even in the absence of ATP.
染色体结构维持（SMC）ATP酶蛋白是同名复合物的重要组成部分，对于跨细菌、古细菌和真核生物等多种生命形式的DNA空间组织至关重要。有人提出，在细菌中，SMC复合物通过环挤压促进DNA压缩，并有助于子代类核的分离。在本文中，通过使用一系列方法，包括传统生化方法以及先进的单分子技术，研究了微小脲原体的SMC ATP酶蛋白的特性。我们的研究结果揭示了该蛋白与其在枯草芽孢杆菌中被广泛研究的同源物相比的独特特性。值得注意的是，我们的结果表明，即使在没有ATP的情况下，微小脲原体Smc ATP酶也能促进DNA压缩。
