Sousa Ana Laura, Tranfield Erin M
Electron Microscopy Facility, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
VIB BioImaging Core - Ghent, VIB Center for Inflammation Research, Ghent, Belgium.
Methods Mol Biol. 2025;2890:151-167. doi: 10.1007/978-1-0716-4326-6_8.
Transmission electron microscopy is an imaging technique that visualizes the ultrastructure of samples, revealing not just the structures of interest but also the context occurring around the structures of interest. When the different approaches to transmission electron microscopy are understood and the right technique is applied to address a biological question, this imaging technique can be extremely useful for a broad diversity of samples and biological questions, including investigations into influenza A infection. In this chapter, three protocols (negative staining, chemical processing, and high-pressure freezing-freeze substitution) routinely used in the Electron Microscopy Facility at the Instituto Gulbenkian de Ciência will be presented.
透射电子显微镜术是一种成像技术，可将样品的超微结构可视化，不仅能揭示感兴趣的结构，还能呈现该结构周围的环境。当理解了透射电子显微镜术的不同方法并应用正确的技术来解决生物学问题时，这种成像技术对于各种各样的样品和生物学问题都极为有用，包括对甲型流感感染的研究。在本章中，将介绍古尔班基安科学研究所电子显微镜设施中常规使用的三种方法（负染色、化学处理和高压冷冻-冷冻置换）。
