Effects of Nickel Bioaccumulation on Hematological, Biochemical, Immune Responses, Neuroinflammatory, Oxidative Stress Parameters, and Neurotoxicity in Rats.

Nickel (Ni) exposure is linked to numerous health issues, including dermatitis, immunotoxicity, and cancer. Emerging evidence suggests Ni may cross the blood-brain barrier, accumulating in the brain and causing neuroinflammation, oxidative stress, and neuronal apoptosis. Herein, we investigated the effect of Ni exposure through the intraperitoneal route, studying the Ni effect in subacute and chronic toxicity, on various health parameters in Wistar rats. Rats were randomly divided into four groups (n = 10 per group): two groups received a daily intraperitoneal injection of NiCl₂ at a dose of 0.25 mg/kg for subacute (21 days) or chronic (60 days) exposure periods, while the other two groups were treated with NaCl solution (0.9%) as a control for equivalent durations. The study assessed behavioral, biochemical, hematological, immunological, neurobiochemical, and histopathological effects over 21 and 60 days. Neurobehavioral tests, blood and tissue analyses, and organ examinations were conducted. This study demonstrates that Ni bioaccumulation in subacute and chronic exposure has significant health impacts in Wistar rats, including hematological, immunological, biochemical, AchE activity, neuroinflammatory, oxidative stress, and neurobehavioral changes. Chronic exposure results in higher Ni accumulation, particularly in the brain, causing neurotoxicity, inflammation, and behavioral disorders such as anxiety, depression, and memory impairment. The findings highlight the importance of limiting Ni exposure to prevent adverse health effects.