将骨形态发生蛋白-2固定于多孔水凝胶中以在空间上调控骨生成。
Immobilization of BMP-2 in porous hydrogels to spatially regulate osteogenesis.
作者信息
Lou Junzhe, Meyer Charlotte, Chen Anqi, Weitz David A, Mooney David J
机构信息
Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA; Department of Information Technology and Electrical Engineering, ETH Zürich, Zürich, Switzerland.
出版信息
J Control Release. 2025 Mar 10;379:944-950. doi: 10.1016/j.jconrel.2025.01.084. Epub 2025 Jan 31.
Sustained release of bone morphogenetic protein 2 (BMP-2) is used to enhance bone regeneration, but immobilizing BMP-2 in three-dimensional scaffolds could enable spatial regulation of stem cell differentiation and bone formation. Here, we fabricate porous granular hydrogels presenting BMP-2 on the surface to regulate stem cell growth and differentiation. Immobilization of BMP-2 and cell-adhesive ligands is achieved by surface-specific functionalization of microgels, which are jammed to form microporous hydrogels. Varying surface ligand density regulated spreading, proliferation and differentiation of cells. In addition, modulating the distribution of cell-adhesive ligands and BMP-2 allowed spatial control over cell adhesion and osteogenic differentiation.
骨形态发生蛋白2（BMP-2）的持续释放用于促进骨再生，但将BMP-2固定在三维支架中能够实现对干细胞分化和骨形成的空间调控。在此，我们制备了表面呈现BMP-2的多孔颗粒水凝胶，以调控干细胞的生长和分化。通过微凝胶的表面特异性功能化实现BMP-2和细胞黏附配体的固定，微凝胶相互挤压形成微孔水凝胶。不同的表面配体密度可调节细胞的铺展、增殖和分化。此外，调节细胞黏附配体和BMP-2的分布能够对细胞黏附和成骨分化进行空间控制。
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