Qiu Xiaoyuan, Yan Danni, Xu Linghui, Wang Ying, Mao Yi, Yang Cheng, Li Yunxing, Sun Yajuan
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122 China.
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122 China.
Int J Pharm. 2025 Feb 25;671:125284. doi: 10.1016/j.ijpharm.2025.125284. Epub 2025 Jan 30.
Pickering emulsions, stabilized by particulate particles, have emerged as a promising vehicle for topical delivery. Herein, Pickering emulsions stabilized by differently charged spirulina protein isolate - chitosan (SC) composite particles were studied for effective topical delivery of α-Bisabolol (ABS). The composite particles were synthesized via electrostatic assembly of spirulina protein isolate (SPI) and chitosan (CS), and their surface charge was assessed using zeta potential measurements. The Pickering emulsions stabilized by SC composite particles with different charges were all stable over 30 days and had a high encapsulation efficiency for ABS. In vitro skin permeation study revealed that positively charged emulsions significantly increased ABS retention within the skin, predominantly in the stratum corneum layer. The underlying delivery mechanism was further explored using attenuated total reflection Fourier transform infrared spectroscopy. Lastly, the influence of particle concentration and oil phase volume fraction on the topical delivery efficiency was conducted to optimize the Pickering formulations. This study provides insight into the role of particle charge in enhancing topical delivery of Pickering emulsions.
由颗粒稳定的皮克林乳液已成为一种有前景的局部给药载体。在此，研究了由带不同电荷的螺旋藻蛋白分离物 - 壳聚糖（SC）复合颗粒稳定的皮克林乳液用于α-红没药醇（ABS）的有效局部给药。复合颗粒通过螺旋藻蛋白分离物（SPI）和壳聚糖（CS）的静电组装合成，并使用zeta电位测量评估其表面电荷。由不同电荷的SC复合颗粒稳定的皮克林乳液在30天内均稳定，并且对ABS具有高包封效率。体外皮肤渗透研究表明，带正电荷的乳液显著增加了ABS在皮肤内的保留，主要是在角质层。使用衰减全反射傅里叶变换红外光谱进一步探索了潜在的递送机制。最后，研究了颗粒浓度和油相体积分数对局部递送效率的影响，以优化皮克林制剂。本研究深入了解了颗粒电荷在增强皮克林乳液局部递送中的作用。
