Gagnon G, Raymond Y
J Immunol Methods. 1985 Apr 22;78(2):267-9. doi: 10.1016/0022-1759(85)90084-5.
A method for cloning hybridomas is described which involves transfer of single cells with a capillary tubing connected to a suction apparatus. This method enables the efficient recovery of antibody-producing clones of differing abundances from the parent hybridoma cultures. The 4 cell lines submitted to this cloning procedure produced a 100% yield of positive wells upon recloning by the same method.
描述了一种克隆杂交瘤的方法，该方法涉及使用连接到抽吸装置的毛细管转移单细胞。这种方法能够从亲本杂交瘤培养物中高效回收不同丰度的产生抗体的克隆。通过相同方法对提交到该克隆程序的4个细胞系进行再次克隆时，阳性孔的产量达到了100%。
