Kumar Arun, Yadav Pradeep Kumar, Singh Anita
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, U.P, 221005, India.
BMC Plant Biol. 2025 Feb 1;25(1):137. doi: 10.1186/s12870-025-06131-7.
The indiscriminate use of pesticides compromises physiology and metabolism in crops, posing health risks through residue accumulation in edible tissues. Amaranthus hybridus L., a fast growing, nutritionally and medicinally valuable crop was studied here to assess the impact of cypermethrin (CYP) at recommended (R1, 100 ppm) and double dose (R2, 200 ppm) alongside foliar application of jasmonic acid (JA) at 50 µM, 100 µM, and 200 µM concentrations. CYP at R1 dose induced hormesis, while R2 was toxic, elevating the production of ROS molecules (HO, SOR, MDA). JA application upregulated the antioxidant activity of SOD, POD, APX, GST, DHAR, GSH, and proline to alleviate oxidative stress and improve growth indicators, including shoot length, leaf area, chlorophyll content, Fv/Fm ratio, and biomass. JA at 100 µM yielded the highest increase in biomass, 11.52% and 13.7% for R1 and R2 treated plants, respectively and also led to reduced accumulation of CYP residues. The UHPLC-MS analysis of leaf tissue revealed increase in the contents of carotenoids, flavonoids, phenolics, phenylpropanoids, steroids content in the plant group combinedly treated with JA and CYP compared to those treated with CYP alone, indicating a protective and growth-promoting role of JA under pesticide stress conditions. Overall, 100 µM concentration of JA proved to be effective against the stress induced by the either dose of CYP in the study. These insights could offer strategies to reduce pesticide-induced damage in vegetable crops, advancing sustainable agriculture.
农药的滥用会损害作物的生理和新陈代谢，通过可食用组织中的残留积累对健康构成风险。本文研究了一种生长迅速、具有营养和药用价值的作物——杂交苋，以评估氯氰菊酯（CYP）在推荐剂量（R1，100 ppm）和双倍剂量（R2，200 ppm）下，以及在50 μM、100 μM和200 μM浓度的茉莉酸（JA）叶面喷施时的影响。R1剂量的CYP诱导了刺激效应，而R2有毒，会提高活性氧分子（HO、SOR、MDA）的产生。JA的施用上调了超氧化物歧化酶、过氧化物酶、抗坏血酸过氧化物酶、谷胱甘肽S-转移酶、脱氢抗坏血酸还原酶、谷胱甘肽和脯氨酸的抗氧化活性，以减轻氧化应激并改善生长指标，包括茎长、叶面积、叶绿素含量、Fv/Fm比值和生物量。100 μM的JA使生物量增加最多，R1和R2处理的植株分别增加了11.52%和13.7%，还导致CYP残留量减少。对叶片组织的超高效液相色谱-质谱分析表明，与单独用CYP处理的植株相比，JA和CYP联合处理的植株组中类胡萝卜素、黄酮类、酚类、苯丙烷类、甾体含量增加，表明在农药胁迫条件下JA具有保护和促进生长的作用。总体而言，在该研究中，100 μM浓度的JA被证明对两种剂量的CYP诱导的胁迫均有效。这些见解可为减少蔬菜作物中农药诱导的损害提供策略，推动可持续农业发展。
