Napoli J L, Pramanik B C, Williams J B, Dawson M I, Hobbs P D
J Lipid Res. 1985 Mar;26(3):387-92.
An assay based on negative ion chemical ionization mass spectrometry has been developed to quantify retinoic acid in plasma or serum. The lower limit of detection is 75 pg (240 fmol); normal values of retinoic acid can be determined on as little as 40 microliters of human plasma. The plasma concentrations of total retinoic acid in 12 healthy male volunteers taking no medication or vitamin supplementation ranged from 2.8 to 6.6 ng/ml; the mean was 4.9 ng/ml. The assay can be manipulated to measure all-trans-retinoic acid alone; about 75% of retinoic acid in human plasma or rat serum is all-trans-retinoic acid. Both retinol and retinoic acid can be quantified on the same 0.1-ml sample; the concentration of retinoic acid in human plasma or rat serum is at least 150-fold less than that of retinol.
已开发出一种基于负离子化学电离质谱法的检测方法，用于定量血浆或血清中的视黄酸。检测下限为75皮克（240飞摩尔）；视黄酸的正常值可在低至40微升的人血浆中测定。12名未服用药物或维生素补充剂的健康男性志愿者血浆中总视黄酸的浓度范围为2.8至6.6纳克/毫升；平均值为4.9纳克/毫升。该检测方法可调整为仅测量全反式视黄酸；人血浆或大鼠血清中约75%的视黄酸为全反式视黄酸。视黄醇和视黄酸均可在同一份0.1毫升的样品中进行定量；人血浆或大鼠血清中视黄酸的浓度至少比视黄醇低150倍。
