Li Yitong, Mai Yuhe, Jiao Yao, Yuan Yali, Qu Yingdi, Zhang Ye, Wang Muyuan, Zhang Wenji, Lu Xinyu, Lin Zhengdao, Liang Chengtao, Li Junxiang, Mao Tangyou, Xie Chune
Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, China.
Shenzhen Bao'an Traditional Chinese Medicine Hospital, Shenzhen, China.
APMIS. 2025 Feb;133(2):e70001. doi: 10.1111/apm.70001.
The gut microbiota plays a critical role in the occurrence and development of IBS-D, however, IBS-D-associated tongue coating microbiome dysbiosis has not yet been clearly defined. To address this, we analyzed the structure and composition of the tongue coating microbiome in 23 IBS-D patients and 12 healthy controls using 16S rRNA high-throughput sequencing analysis. The 16S rRNA sequencing results revealed that the overall observed OTUs of tongue coating microbiome in IBS-D patients exhibited a significant decrease compared with the healthy controls. Alpha diversity analysis showed that the diversity and community richness were significantly reduced in IBS-D patients, and PCoA revealed a distinct clustering of tongue coating microbiome between the IBS-D patients and healthy controls. Microbial comparisons at the genus level showed that the abundance of Veillonella, Prevotella in IBS-D patients was higher than those in healthy controls, while Streptococcus, Haemophilus, Granulicatella, and Rothia were significantly reduced compared with the healthy volunteers. Functional analysis results showed significant differences in 88 functional metabolic pathways between the IBS-D patients and the healthy controls, including fatty acid biosynthesis. These findings identified the structure, composition, functionality of tongue coating microbiome in IBS-D patients, and hold promise the potential for therapeutic targets during IBS-D management.
肠道微生物群在腹泻型肠易激综合征（IBS-D）的发生和发展中起关键作用，然而，与IBS-D相关的舌苔微生物群失调尚未明确界定。为解决这一问题，我们使用16S rRNA高通量测序分析，对23例IBS-D患者和12名健康对照者的舌苔微生物群的结构和组成进行了分析。16S rRNA测序结果显示，与健康对照相比，IBS-D患者舌苔微生物群的总体观察到的操作分类单元（OTUs）显著减少。α多样性分析表明，IBS-D患者的多样性和群落丰富度显著降低，主坐标分析（PCoA）显示IBS-D患者和健康对照者之间舌苔微生物群有明显的聚类。属水平的微生物比较表明，IBS-D患者中韦荣球菌属、普雷沃菌属的丰度高于健康对照，而与健康志愿者相比，链球菌属、嗜血杆菌属、颗粒链菌属和罗氏菌属显著减少。功能分析结果显示，IBS-D患者与健康对照之间在88条功能代谢途径上存在显著差异，包括脂肪酸生物合成。这些发现确定了IBS-D患者舌苔微生物群的结构、组成和功能，并有望为IBS-D管理中的治疗靶点提供潜力。
