Mahdavinejad Arezou, Shadnia Shahin, Farhadinejad Kiana, Farnam Golrokh, H Shirazi Farshad
Toxicological Research Center, Excellence Center of Clinical Toxicology, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2024 Aug 20;23(1):e146675. doi: 10.5812/ijpr-146675. eCollection 2024 Jan-Dec.
Alprazolam, a commonly prescribed benzodiazepine, poses risks of toxicity and severe withdrawal symptoms. There is an urgent need for a rapid and sensitive diagnostic method for detecting alprazolam poisoning.
This study aimed to detect alprazolam poisoning through Fourier-transform infrared (FTIR) analysis of saliva, addressing the need for a quick, cost-effective, and sensitive diagnostic method for poison control and differential diagnosis.
Saliva samples were collected from 45 individuals with benzodiazepine toxicity, therapeutic consumption, and normal health status, as well as from a control group. The samples were analyzed using FTIR spectroscopy. The resulting spectra were processed with OriginPro software, and statistical analyses were performed using receiver operating characteristic (ROC) and analysis of variance (ANOVA).
The average age of the studied population was approximately 45 years, with women being the most affected by poisoning. Fourier-transform infrared analysis revealed significant differences in the structure of lipids between poisoned individuals, therapeutic receivers, and healthy individuals (P < 0.0001).
Fourier-transform infrared analysis of saliva is a fast and accurate method for diagnosing alprazolam poisoning within minutes, enabling prompt and appropriate treatment during critical life-threatening situations. This non-invasive technique has the potential to guide treatment staff toward effective treatment options.
阿普唑仑是一种常用的苯二氮䓬类药物，存在毒性风险和严重的戒断症状。迫切需要一种快速、灵敏的诊断方法来检测阿普唑仑中毒。
本研究旨在通过对唾液进行傅里叶变换红外光谱（FTIR）分析来检测阿普唑仑中毒，以满足对毒物控制和鉴别诊断的快速、经济高效且灵敏的诊断方法的需求。
从45名患有苯二氮䓬类药物中毒、治疗性用药和健康状况正常的个体以及一个对照组中收集唾液样本。使用FTIR光谱对样本进行分析。所得光谱用OriginPro软件进行处理，并使用受试者工作特征曲线（ROC）和方差分析（ANOVA）进行统计分析。
研究人群的平均年龄约为45岁，女性受中毒影响最大。傅里叶变换红外光谱分析显示，中毒个体、治疗性用药个体和健康个体之间的脂质结构存在显著差异（P < 0.0001）。
唾液的傅里叶变换红外光谱分析是一种在数分钟内诊断阿普唑仑中毒的快速、准确方法，能够在危及生命的关键情况下实现及时、恰当的治疗。这种非侵入性技术有可能指导治疗人员选择有效的治疗方案。
