Design and Validation of an Open-Close Device for Integrated Environmental DNA Sampling Detects A Depth Gradient in Indian Ocean Deep-Sea Fish Assemblages.

Advances in methods for collecting environmental DNA (eDNA) are revolutionizing biomonitoring capabilities. The goal of this study was to leverage existing survey technology to design and test an eDNA sampler that captures an integrated eDNA sample over the length of a deep-water transect. We manufactured a 300 × 100 × 100 mm mountable, open-ended box made of high-density polyethylene that could be attached to the frame of a preexisting deep tow camera system. The box (OCD; open-close device) was equipped with an actuator that attached to hinged doors at both ends, enabling it to be opened and closed remotely at depths up to 6000 m through preexisting communications, thereby exposing the internal chamber to the surrounding water upon activation. A sterile active carbon sponge was inserted into the internal chamber for eDNA capture during each deployment. The OCD sampler was field tested during a voyage to the Gascoyne Marine Park region off northwest Australia. We compared three different methods for processing the captured eDNA from the sampler: filtering OCD water, extracting eDNA from sponge pieces, and filtering sponge rinse water. Using fish as our example organism, we also compared the identities of fishes from eDNA detections with bottom trawl survey data collected during the same survey, and the known regional species pool, to confirm the eDNA identifications were plausible. A large number of fishes (193 taxa, from 87 families) were detected, and the majority were found within their expected depth ranges (> 75%), and in the trawl catches (60%). We discuss design and manufacturing lessons, ideas for increased eDNA capture efficiency for improved methodologies in sample processing, and how to establish appropriate field controls. We also discuss how this technology could advance our scientific understanding in ocean studies in terms of ecological metrics provided and the trade-offs compared to other sampling tools.